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Gargi Kulkarni
Researcher at California Institute of Technology
Publications - 17
Citations - 800
Gargi Kulkarni is an academic researcher from California Institute of Technology. The author has contributed to research in topics: Methanosarcina barkeri & Hydrogenase. The author has an hindex of 12, co-authored 16 publications receiving 656 citations. Previous affiliations of Gargi Kulkarni include University of Illinois at Urbana–Champaign & Howard Hughes Medical Institute.
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Journal ArticleDOI
Energy Conservation via Hydrogen Cycling in the Methanogenic Archaeon Methanosarcina barkeri.
TL;DR: Characterizing a series of hydrogenase mutants to provide direct evidence of H2 cycling adds to a short list of mechanisms for generating a transmembrane electrochemical gradient that is likely to be widespread, especially among anaerobic microorganisms.
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New methods for tightly regulated gene expression and highly efficient chromosomal integration of cloned genes for Methanosarcina species.
TL;DR: A series of plasmid vectors that utilize the site-specific recombination system for construction of reporter gene fusions and for tetracycline regulated expression of cloned genes are reported.
Journal ArticleDOI
Covalently linked hopanoid-lipid A improves outer-membrane resistance of a Bradyrhizobium symbiont of legumes
Alba Silipo,Giuseppe Vitiello,Djamel Gully,Luisa Sturiale,Clémence Chaintreuil,Joël Fardoux,Daniel Gargani,Hae In Lee,Gargi Kulkarni,Nicolas Busset,Roberta Marchetti,Angelo Palmigiano,Herman Moll,Regina Engel,Rosa Lanzetta,Luigi Paduano,Michelangelo Parrilli,Woo Suk Chang,Woo Suk Chang,Otto Holst,Dianne K. Newman,Domenico Garozzo,Gerardino D'Errico,Eric Giraud,Antonio Molinaro +24 more
TL;DR: It is demonstrated that a photosynthetic Bradyrhizobium strain, symbiont of Aeschynomene legumes, synthesizes a unique LPS bearing a hopanoid covalently attached to lipid A that reinforces the stability and rigidity of the outer membrane.
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Hydrogen is a preferred intermediate in the energy-conserving electron transport chain of Methanosarcina barkeri
Gargi Kulkarni,Donna M. Kridelbaugh,Donna M. Kridelbaugh,Adam M. Guss,Adam M. Guss,William W. Metcalf +5 more
TL;DR: The data suggest that the rapid enzymatic turnover of hydrogenases may allow a competitive advantage via faster growth rates in this freshwater organism and suggest that M. barkeri does have the flexibility to use the Fpo-dependent electron transport chain when needed.
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The general stress response factor EcfG regulates expression of the C-2 hopanoid methylase HpnP in Rhodopseudomonas palustris TIE-1
TL;DR: This is the first dissection of the regulation of hpnP, the gene encoding the C-2 hopanoid methylase, at the molecular level, and it is demonstrated that EcfG, the general stress response factor of alphaproteobacteria, regulates expression of hPNP under a variety of challenges, including high temperature, pH stress, and presence of nonionic osmolytes.