The present invention provides systems, methods, screens, reagents and kits for optical system analysis of cells to rapidly determine the distribution, environment, or activity of fluorescently labeled reporter molecules in cells for the purpose of screening large numbers of compounds for those that specifically affect particular biological functions.

A system for cell-based screening

The present invention relates to new organic phosphorus containing compounds which have been found to have surface active properties. The present compounds are new phosphinic acids of alkylated benzene hydrocarbons and amine derivatives or salts thereof. They may be generally represented by...

Organic Phosphorus Compounds

The assay devices, assay systems and device components of this invention comprise at least two opposing surfaces disposed a capillary distance apart, at least one of which is capable of immobilizing at least one target ligand or a conjugate in an amount related to the presence or amount of target ligand in the sample from a fluid sample in a zone for controlled fluid movement to, through or away the zone. The inventive device components may be incorporated into conventional assay devices with membranes or may be used in the inventive membrane-less devices herein described and claimed. These components include flow control elements, measurement elements, time gates, elements for the elimination of pipetting steps, and generally, elements for the controlled flow, timing, delivery, incubation, separation, washing and other steps of the assay process.

Diagnostic devices and apparatus for the controlled movement of reagents without membranes

Creatine: biosynthesis, regulation, and function.

1,160,436. Substituted amino-dialkylene phosphonic acid derivatives. MONSANTO CO. 3 Aug., 1966, No. 34861/66. Addition to 1,023,785. Heading C2C. [Also in Division C5] Esters of the general formula where R contains from 4 to 30 carbon atoms, is substituted or unsubstituted, and is an alicyclic group, an aryl group, an alkaryl group or an aralkyl group, R 1 , R 2 , R 3 and R 4 are hydrogen, aliphatic hydrocarbyl, alicyclic, aryl, alkaryl, aralkyl or heterocyclic groups which are optionally substituted and contain from 4 to 30 carbon atoms, X is optionally substituted and is a C 1-30 aliphatic hydrocarbyl group, an aryl, alkaryl, aralkyl or alicyclic group or a cation, are prepared by reacting a primary amine, an aldehyde and a diester of phosphorous acid, or orthophosphorous acid, or by hydrolysis of an appropriate phosphonate ester with HCl or HBr, or by reacting an amine with a carbonyl compound to form an imine and reacting this with a dialkyl phosphite. The compounds exhibit deflocculating and surfactancy proper. ties.

Organic phosphorus compounds

Method and compositions are provided for performing homogeneous immunoassays. The method involves having a signal producing system, which provides a detectable signal, which system includes a macromolecular member. The determination of the analyte, which is a member of a specific binding pair consisting of a ligand and its homologous receptor, is performed by creating an extensive matrix in the assay medium by having in the assay medium in addition to the analyte, ligand labeled with one of the members of the signal producing system, antiligand either present as the analyte or added, a polyvalent receptor for antiligand, the macromolecular member of the signal producing system, and any additional members of the signal producing system. The labeled ligand, antiligand, and polyvalent receptor for the antiligand create a matrix which modulates, e.g. inhibits, the approach of the macromolecular member of the signal producing system to the labeled ligand. The extent and degree of formation of the matrix is dependent upon the concentration of the analyte in the medium. By comparing the signal from an assay medium having an unknown amount of analyte, with a signal obtained from an assay medium having a known amount of analyte, the amount of analyte in the unknown sample may be determined qualitatively or quantitatively. Kits are provided having predetermined amounts of the various reagents to allow for enhanced sensitivity of the method.

Double antibody for enhanced sensitivity in immunoassay

On the specificity of creatine kinase. New glycocyamines and glycocyamine analogs related to creatine.

Tautomeric preferences among glycocyamidines

Homogeneous enzyme immunoassay for proteins employing beta-galactosidase.

A device is disclosed for use in a chromatographic system wherein a component of a mixture is partitioned between a liquid phase and an immobile phase. The device comprises at least one strip of a bibulous material. In the chromatographic system the component traverses at least a portion of the strip. The strip generally has a longitudinal edge substantially corresponding to the direction of traverse of the component. The longitudinal edge has the characteristic of substantially the same rate of traversal by the component along this edge when compared to the rate of traversal of the component along the body of the strip. The strips are prepared from a sheet of a bibulous material by non-deformative or non-compressive cutting of the sheet. The preferred cutting means is a laser beam.

Gerald L. Rowley

Papers

Double antibody for enhanced sensitivity in immunoassay

On the specificity of creatine kinase. New glycocyamines and glycocyamine analogs related to creatine.

Tautomeric preferences among glycocyamidines

Homogeneous enzyme immunoassay for proteins employing beta-galactosidase.

Chromatographic strip having non-compressed edges