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Gianluca Petris
Researcher at University of Trento
Publications - 32
Citations - 925
Gianluca Petris is an academic researcher from University of Trento. The author has contributed to research in topics: Endoplasmic reticulum & Genome editing. The author has an hindex of 12, co-authored 25 publications receiving 582 citations. Previous affiliations of Gianluca Petris include Laboratory of Molecular Biology & International Centre for Genetic Engineering and Biotechnology.
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Journal ArticleDOI
A highly specific SpCas9 variant is identified by in vivo screening in yeast.
Antonio Casini,Michele Olivieri,Gianluca Petris,Claudia Montagna,Giordano Reginato,Giulia Maule,Francesca Lorenzin,Davide Prandi,Alessandro Romanel,Francesca Demichelis,Alberto Inga,Anna Cereseto +11 more
TL;DR: A yeast-based assay to identify optimized Streptococcus pyogenes Cas9 (SpCas9) variants that enables simultaneous evaluation of on- and off-target activity is developed and evoCas9, a variant that has fidelity exceeding both wild-type and rationally designed Cas9 variants, is generated.
Journal ArticleDOI
Hit and go CAS9 delivered through a lentiviral based self-limiting circuit
Gianluca Petris,Antonio Casini,Claudia Montagna,Francesca Lorenzin,Davide Prandi,Alessandro Romanel,Jacopo Zasso,Luciano Conti,Francesca Demichelis,Anna Cereseto +9 more
TL;DR: A Self-Limiting Cas9 circuit for Enhanced Safety and specificity (SLiCES) which consists of an expression unit for Streptococcus pyogenes Cas9, a self-targeting sgRNA and a second sg RNA targeting a chosen genomic locus results in increased genome editing specificity by controlling Cas9 levels.
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VSV-G-Enveloped Vesicles for Traceless Delivery of CRISPR-Cas9.
Claudia Montagna,Gianluca Petris,Antonio Casini,Giulia Maule,Gian Marco Franceschini,Ilaria Zanella,Luciano Conti,Francesca Arnoldi,Oscar R. Burrone,Lorena Zentilin,Serena Zacchigna,Serena Zacchigna,Mauro Giacca,Mauro Giacca,Anna Cereseto +14 more
TL;DR: The authors developed vesicles (VEsiCas) carrying CRISPR-SpCas9 ribonucleoprotein complexes (RNPs) that are efficiently delivered into target cells through the fusogenic glycoprotein of the vesicular stomatitis virus (VSV-G).
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Allele specific repair of splicing mutations in cystic fibrosis through AsCas12a genome editing.
Giulia Maule,Antonio Casini,Claudia Montagna,Anabela S. Ramalho,Kris De Boeck,Zeger Debyser,Marianne Carlon,Gianluca Petris,Gianluca Petris,Anna Cereseto +9 more
TL;DR: A gene therapy approach using the programmable nuclease AsCas12a to correct a splicing mutation in CFTR, and show efficient repair of the mutation and recovery of CFTR function in patient-derived organoids and airway epithelial cells.
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Tobacco Etch Virus protease: A shortcut across biotechnologies
TL;DR: TEVp is a unique endopeptidase largely exploited in biotechnology from industrial applications to in vitro and in vivo cellular studies, and some of its multiple applications are described.