Grzegorz Piszczek

TL;DR: A method for automated peak assignment based on peak-shape analysis via singular value decomposition in combination with detailed least-squares modeling of local pre- and postinjection baselines is described, which results in improved detection limits for high-affinity or low-enthalpy binding reactions and significantly higher precision of the derived thermodynamic parameters.

TL;DR: The present communication reviews principles and strategies of global analysis for ITC and its extension to GMMA in SEDPHAT and introduces a new graphical tool for aiding experimental design by surveying the concentration space and generating simulated data sets, which can be subsequently statistically examined for their information content.

TL;DR: E engineered variants of the photoconvertible Eos fluorescent protein are reported that fluoresce and photoconverts normally in heavily fixed samples, enabling correlative super-resolution fluorescence imaging and high-quality electron microscopy.

TL;DR: Results show that nucleic acid structures, in addition to sequences, influence the binding and activity of regulatory proteins in genome-wide R-loops.

TL;DR: The first crystal structure of TTL is presented, defining the structural scaffold upon which the diverse TTL-like family of tubulin-modifying enzymes is built and possibly modulating the partition oftubulin between monomeric and polymeric forms.