Harald F. Hess

TL;DR: This work introduced a method for optically imaging intracellular proteins at nanometer spatial resolution and used this method to image specific target proteins in thin sections of lysosomes and mitochondria and in fixed whole cells to image retroviral protein Gag at the plasma membrane.

TL;DR: Three-dimensional super-resolution fluorescence microscopy is used to map nanoscale protein organization in focal adhesions and reveals talin’s polarized orientation, indicative of a role in organizing the focal adhesion strata.

TL;DR: This work created spatially resolved maps of single-molecule motions by imaging the membrane proteins Gag and VSVG, and obtained several orders of magnitude more trajectories per cell than traditional single-particle tracking enables.

TL;DR: iPALM closes the gap between electron tomography and light microscopy, enabling both molecular specification and resolution of cellular nanoarchitecture.

TL;DR: Improved methods are summarized and the circuitry of a large fraction of the brain of the fruit fly Drosophila melanogaster is presented, reducing the effort needed to answer circuit questions and providing procedures linking the neurons defined by the analysis with genetic reagents.