Hana Vlčková

TL;DR: UHPLC has recently become a wide-spread analytical technique in many laboratories which focus on fast and sensitive bio-analytical assays and the key advantages are the increased speed of analysis, higher separation efficiency and resolution, higher sensitivity and much lower solvent consumption as compared to other analytical approaches.

TL;DR: An overview of UHPLC in HILIC mode, including the importance of the final sample diluent, a discussion on available stationary phases and approaches to detection, and a comparison of performance with other chromatographic modes are presented in this paper.

TL;DR: Simvastatin and atorvastarin as the most widely used statins in clinical treatment and their hydroxy-acid/lactone forms were determined by means of UPLC in connection with triple quadrupole mass spectrometer.

TL;DR: Combination of fast MEPS method together with quick UHPLC-MS/MS was used for the determination of atorvastatin and its two metabolites in serum obtained from patients with familiar hypercholesterolemia.

TL;DR: The UHPLC-MS/MS method was validated and demonstrated good linearity in range 5-500 nmol/l (r(2)>0.9990) for plasma and urine samples and method recovery was ranged within 97-109% for plasma samples and 92-101% for the urine samples.