Heiner Niemann

TL;DR: A dual role is assigned to SNAP-25 and it is suggested that its nine C-terminal amino acids are directly involved in coupling the calcium sensor to the final step in exocytosis.

TL;DR: This study found that syntaxin 3, SNAP23, and a newly identified VAMP/brevin, tetanus neurotoxin (TeNT)-insensitive VAMP (TI-VAMP), are insensitive to clostridial NTs and are suggested to participate in exocytotic processes at the apical plasma membrane of epithelial cells and, more generally, domain-specificExocytosis in clostidial NT-resistant pathways.

TL;DR: The complete sequence of botulinum neurotoxin type A (BoNT/A; 1,296 amino acid residues, Mr = 149,425) and a partial sequence ofotoxin type E (273 amino acids residues) as deduced from the corresponding nucleotide sequences of the chromosomally located structural genes are established.

TL;DR: Overlapping peptides together covering the entire tetanus toxin molecule were synthesized in Escherichia coli and identified by monoclonal antibodies, indicating that the neurotoxins from C. tetani and C. botulinum are derived from a common ancestral gene.

TL;DR: It is demonstrated that cleavage of SNAP‐25 occurs between Arg198 and Ala199, depends on the presence of regions Asn93 to Glu145 and Ile156 to Met202, and requires about 1,000‐fold higher L chain concentrations in comparison with BoNT/A and BoNT /E.