H
Helga Hinze
Researcher at University of Freiburg
Publications - 18
Citations - 591
Helga Hinze is an academic researcher from University of Freiburg. The author has contributed to research in topics: Sulfite & Dehydrogenase. The author has an hindex of 12, co-authored 18 publications receiving 585 citations.
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Journal ArticleDOI
Isolation and Properties of Two Inhibitors of Proteinase B from Yeast
TL;DR: Two protein inhibitors of the tryptophan synthase inactivating yeast proteinase B were purified from boiled bakers' yeast extract and are very stable in the pH range of 1 to 10, but they are easily destroyed by incubation with proteinase A and, less effectively, withproteinase B.
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Effects of glucose and nitrogen source on the levels of proteinases, peptidases, and proteinase inhibitors in yeast.
TL;DR: In Saccharomyces cerevisiae harvested from early exponential growth on glucose-containing media, the specifc activities of proteinases A and B, carboxypeptidase Y, and the inhibitors IA, IB, IC of these three proteinases are found to be 10-30% of the specific activities observed in media without glucose, containing acetate as a carbon source.
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Analysis of the energy metabolism after incubation of Saccharomyces cerevisiae with sulfite or nitrite.
Helga Hinze,Helmut Holzer +1 more
TL;DR: It is concluded that the effects of sulfite or nitrite on ATP, ADP and inorganic phosphate are the result of inhibition of glyceraldehyde-3-phosphate dehydrogenase and not of inhibited phosphorylation processes in the mitochondria.
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Assay of phosphoenolpyruvate car☐ykinase in crude yeast extracts
TL;DR: Observations demonstrated that the assay provides a quantitative measure of phosphoenolpyruvate car☐ykinase activity in crude extracts, and showed that the disappearance of NADH was shown to be accompanied by stoichiometric accumulation of malate.
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Mechanism of sulfite action on the energy metabolism of Saccharomyces cerevisiae
TL;DR: Sulfite causes reduction of oxygen consumption of glucose-starved yeast at pH 3.6 which coincides with ATP depletion, and formation of adducts between sulfite and aldehydes contributes to the inhibition of enzymatic reactions as shown with alcohol dehydrogenase.