Showing papers in "Biochimica et Biophysica Acta in 1986"

TL;DR: A modified form of the dim methylmethylene blue assay is described that has improved specificity for sulphated glycosaminoglycans, and it is shown that in conjunction with specific polysaccharidases, the dimethylmethyleneblue assay can be used to quantitate individual sulphated sugarcans.

TL;DR: Freeze-fracture electron microscopy revealed that vesicles produced at very high lipid concentrations exhibit size distributions and extent of multilamellar character comparable to systems produced at lower lipid levels.

TL;DR: Inhibition of mitochondria ATPase likely accounts for the cytotoxicity of Rhodamine 123, a sensitive and specific probe of delta psi in isolated mitochondria at concentrations which do not inhibit mitochondrial function.

TL;DR: Although pore formation is apparently involved in the toxicity of amphotericin B and nystatin, it is not the sole factor which contributes to cell death, since K+ leakage induced by these antibiotics is separate from their lethal action.

TL;DR: When applied to the L and M subunits of Rhodopseudomonas sphaeroides, five helices were predicted, which is consistent with the three-dimensional X-ray crystal structure.

TL;DR: CS-514 markedly reduced serum cholesterol levels in dogs, monkeys and rabbits, including Watanabe heritable hyperlipidemic rabbits, an animal model for familial hypercholesterolemia in man, but did not reduce those in rats and mice.

TL;DR: It is demonstrated here that adriamycin can be rapidly and efficiently accumulated into egg phosphatidylcholine and egg PC-cholesterol LUVs in response to a transmembrane pH gradient (interior acidic) in the absence of ionophores.

TL;DR: The manner in which cytolytic peptides interact with plasma membranes of eukaryotic cells, particularly the membranes of erythrocytes, has been discussed with emphasis on melittin, thiolactivated lysins and staphylococcal alpha-toxin.

TL;DR: A regulatory role for β 2 -glycoprotein-I is suggested in the pathway of blood coagulation because it causes a reduction of the prothrombinase binding sites of these coagulations factors to platelets or phospholipid vesicles.

TL;DR: It was found that DHIC-derived monomer units comprise only approx.

TL;DR: A simple assay for detecting antifungal compounds utilizing impregnated paper discs on agar to inhibit mycelial spread of an indicator organism, Trichoderma reesei, is developed and purified to apparent homogeneity two antIFungal proteins from dehusked barley grain.

TL;DR: The ability of various carbohydrates to prevent leakage of a small, water-soluble marker from unilamellar liposomes during freeze-drying has been investigated and compared with the ability of trehalose to prevent such leakage as mentioned in this paper.

TL;DR: Compared to malonaldehyde, 4-hydroxynonenal exhibits a much higher capacity to modify LDL and it is believed that this aldehyde is a more likely candidate for being responsible for LDL modification under in vivo lipid peroxidation conditions.

TL;DR: The primary determinant of metallothionein protection appears to be metal release and subsequent uptake by the membranes, a protein known to be induced by various prooxidant conditions.

TL;DR: The present study provides the first evidence that apolipoprotein A-IV can play a role in reverse cholesterol transport as was suggested on the basis of high concentrations of this apoledipoprotein in nonlipoprotein form in plasma and extracellular fluid.

TL;DR: Patients with rheumatoid arthritis have altered protein patterns in their serum and synovial fluid which influences the antioxidant activity of these fluids and prolonged incubations with trypsin reduced the iron-binding capacity of transferrin and decreased the ferroxidase and antioxidant properties of caeruloplasmin.

TL;DR: Findings indicate that hormone-sensitive lipase catalyzes the hydrolysis of triacylglycerol in the rate-limiting step of adipose tissues lipolysis, and of the resulting diacyl glycerol, whereas the action of monoacyl Glycerol lipase is required in the final hydrolytic step of the 2-monoacy lglycerols produced.

TL;DR: A rough correlation between the halfwave reduction potential (E1/2) of the quinone used for reconstitution and the apparent free energy of the state P+Q- relatively to P is found, indicating that the intermediate is probably not simply an activated form of P-Q-.

TL;DR: It is concluded that Percoll, although particulate, is captured by pinocytosis, and the pattern of inhibition of uptake of polystyrene particles suggests that there is no radical discontinuity between pinocytic and phagocytic uptake, but that the contribution of phagocytetosis steadily increases with increasing particle diameter.

TL;DR: A systematic study of the distribution and metabolism of nitroxide in biological systems provides a reasonably coherent picture of some aspects of the interactions between nitroxides and cells.

TL;DR: The results suggest that cyclic electron flow around PS II occurs under normal physiological conditions and is especially pronounced in chlorophytes, and suggest that a long-lived quencher of fluorescence is formed at moderate to continuous irradiance levels.

TL;DR: It is concluded that melittin induces a reorganization of lipid assemblies which can involve different processes, depending on experimental conditions: vesicularization of multibilayers; fusion of small lipid vesicles; fragmentation into discs and micelles.

TL;DR: A comparison of the stabilizing effect of sucrose with that of trehalose and glucose showed that the stabilization is not sugar-specific but is a general property of saccharides.

TL;DR: A quenched-flow apparatus and a newly developed automated syringe system have been used to measure initial rates of d −[14C]glucose transport into human red blood cells at temperatures ranging from 0° to 53°C as discussed by the authors.