Application of conducting polymers to biosensors.

Polymers in sensor applications

The various types of biosensors such as enzyme-based, tissue-based, immunosensors, DNA biosensors, thermal and piezoelectric biosensors have been deliberated here to highlight their indispensable applications in multitudinous fields. Some of the popular fields implementing the use of biosensors are food industry to keep a check on its quality and safety, to help distinguish between the natural and artificial; in the fermentation industry and in the saccharification process to detect precise glucose concentrations; in metabolic engineering to enable in vivo monitoring of cellular metabolism. Biosensors and their role in medical science including early stage detection of human interleukin-10 causing heart diseases, rapid detection of human papilloma virus, etc. are important aspects. Fluorescent biosensors play a vital role in drug discovery and in cancer. Biosensor applications are prevalent in the plant biology sector to find out the missing links required in metabolic processes. Other applications are involved in defence, clinical sector, and for marine applications.

Biosensors and their applications - A review.

Immobilization strategies to develop enzymatic biosensors

A review on protein–phenolic interactions and associated changes

Sensitivity and selectivity of electrochemical enzyme sensors for inhibitor determination.

Acetylcholinesterase (ChE) sensor based on Prussian blue (PB) modified electrode was developed and tested for the detection of organophosphorus and carbamic pesticides. The signal of the sensor was generated in PB mediated oxidation of thiocholine recorded at+200 mv in DC mode. ChE from electric eel was immobilized by cross-linking with glutaraldehyde in the presence of bovine serum albumin (BSA) on the surface of screen-printed carbon electrode covered with PB and Nafion. The content of the surface layer (specific enzyme activity, Nafion and BSA amounts) was optimized to establish high and reliable response toward the substrate and ChE inhibitors. The ChE/PB sensor makes it possible to detect Aldicarb, Paraoxon and Parathion-Methyl with limits of detection 30, 10 and 5 ppb, respectively (incubation 10 min). The feasibility of practical application of the ChE/PB sensor developed for the monitoring of degradation of the pesticides in wine fermentation was shown. To diminish matrix interferences, the electrolysis of the grape juice with Al anode and evaporation of ethanol were suggested, however the procedures decrease the sensitivity of pesticide detection and stability of the sample tested.

/pdf/acetylcholinesterase-sensor-based-on-screen-printed-carbon-derw0nxzsh.pdf

Herman Budnikov

Papers

Sensitivity and selectivity of electrochemical enzyme sensors for inhibitor determination.

Acetylcholinesterase sensor based on screen-printed carbon electrode modified with prussian blue

Amperometric biosensors based on nafion coated screen-printed electrodes for the determination of cholinesterase inhibitors.

Acetylcholinesterase sensors based on gold electrodes modified with dendrimer and polyaniline: A comparative research

Comparative investigation of electrochemical cholinesterase biosensors for pesticide determination