Showing papers by "Hiroki Kondo published in 2006"
TL;DR: The methylation-specific PCR of methylated genome containing 10-times excess of unmethylated one could be detected by the identical electrochemical signals from the two DNA probes under the settled optimum hybridization conditions.
28 citations
TL;DR: DNA duplex regions of a spot on a DNA microarray were successfully visualized by scanning electrochemical microscopy coupled with ferrocenylnaphthalene diimide as an electrochemically active DNA hybrid indicator.
18 citations
TL;DR: In this article, the chemical structures of SiO2/Si interfaces were studied by photoelectron spectroscopy using high-brilliance soft X-ray with photon energy ranging from 500 to 1500 eV at Super Photon ring 8 GeV(SPring-8) and it is able to probe a depth of about 1.2 to 3 nm with energy resolution of 100 meV.
Abstract: The chemical structures of SiO2/Si interfaces were studied by photoelectron spectroscopy using high-brilliance soft X-ray with photon energy ranging from 500 to 1500 eV at Super Photon ring 8 GeV(SPring-8) and it is able to probe a depth of about 1.2 to 3 nm with energy resolution of 100 meV. On the other hand, high-brilliance hard X-ray with photon energy ranging from 6 to 10 keV is able to probe a depth of about 8.5 to 12.5 nm with energy resolution of 100 meV. Hard photoelectron spectroscopy are particularly useful for studying the composition and the chemical structure of transition layer at high-k dielectric/silicon interface.
11 citations
TL;DR: Biotin carboxyl carrier protein is one subunit or domain of biotin‐dependent enzymes that has serine replaced for the methionine C‐terminal to the lysine in Archaeon Sulfolobus tokodaii, indicating that the substrate specificity is different between the two organisms.
11 citations
TL;DR: The K(m) values of the resulting mutant, A43G, for substrates, were smaller than those of the wild type, suggesting that the residue in position 43 of BPL plays an important role in substrate binding.
9 citations
TL;DR: In this article, an initial stage of TiN growth on SiO2 substrates by ultrahigh-vacuum chemical vapor deposition with TiCl4 and NH3 as source materials was investigated.
Abstract: We have investigated an initial stage of titanium nitride (TiN) growth on SiO2 substrates by ultrahigh-vacuum chemical vapor deposition with TiCl4 and NH3 as source materials. The behaviors of nucleation and grain growth of TiN have been clarified by atomic force microscopy and transmission electron microscopy. It was found that TiN film formation at an initial stage consists of three stages, which are characteristic of the lateral and subsequent vertical growth processes of grains. Deposition time dependence of the lateral growth of TiN grains clearly indicates that a process at 550°C is limited by the surface reaction, irrespective of the TiCl4 flow rate and a pretreatment for the substrates before the deposition. The pretreatment affects in the generation of additional nucleation sites on the SiO2 surface but does not affect the mechanisms of nucleation and grain growth.
6 citations
4 citations
01 Nov 2006
TL;DR: DNase I in one microl of the water could quantitate electrochemically with the detection limit of 0.01 units (ca. 20 pg) by using the ferrocenyl oligonucleotide-immobilized electrode prepared by thiolated oligon nucleotide and ferroenyl carbodiimide as a simple labeling reagent of redox unit.
Abstract: DNase I in one microl of the water could quantitate electrochemically with the detection limit of 0.01 units (ca. 20 pg) by using the ferrocenyl oligonucleotide-immobilized electrode prepared by thiolated oligonucleotide and ferrocenyl carbodiimide as a simple labeling reagent of redox unit.
4 citations
01 Nov 2006
TL;DR: Large hypochromic effect of these derivatives upon binding to the tetraplex DNA suggested that the binding mode ofThese derivatives might contribute the stacking interaction between the naphthalene diimide and guanine tetraPlex planes.
Abstract: Spectrophotometric binding studies of a series of the naphthalene diimide derivatives, 1-7, carrying different chains with a human telomere oligonucleotide, d(TTAGG)(4) was carried out in 0.1 M AcOK-AcOH buffer (pH 5.6) and 0.1 M KCl. Under this condition, this DNA could exist as the mixture of two-type tetraplex structures and these derivatives could bind to this DNA with strong affinity of 10(6) M(-1). The effect of the linker chain is not so large in those binding affinity, but the ligand 5 having piperazine skeleton in the linker chain had relative higher affinity for this tetraplex DNA than other derivatives. Large hypochromic effect of these derivatives upon binding to the tetraplex DNA suggested that the binding mode of these derivatives might contribute the stacking interaction between the naphthalene diimide and guanine tetraplex planes.
1 citations
15 May 2006
TL;DR: In this article, it was found that patterning of Ge and SiGe layers on Si(001) substrates principally leads to the strain anisotropy, and a clear difference of strain relaxation mechanism depending on the dislocation introduction was confirmed.
Abstract: It was found that patterning of Ge and SiGe layers on Si(001) substrates principally leads to the strain anisotropy. The striped mesa structure readily induces elastic relaxation of the strained Ge and SiGe. Furthermore, a clear difference of strain relaxation mechanism depending on the dislocation introduction was confirmed. Introduction of 60deg dislocations is sensitive to the shape of patterned region, resulting in the anisotropic strain. On the other hand, the pure-edge dislocation network explicitly leads to isotropic strain relaxation even in the miniaturized region