Hitoshi Nakayashiki

TL;DR: A pHANNIBAL-like silencing vector, pSilent-1, for ascomycete fungi, which carries a hygromycin resistance cassette and a transcriptional unit for hairpin RNA expression with a spacer of a cutinase gene intron from the rice blast fungus Magnaporthe oryzae, shows higher efficiency in silencing of the eGFP gene.

TL;DR: It is proposed that the multiple translocation of AVR-Pita may be associated with its frequent loss and recovery mediated by its transfer among individuals in asexual populations, which implies that the high mobility of A VR-Pitas is a key mechanism accounting for the rapid adaptation toward Pita.

TL;DR: Two RNA silencing‐related phenomena, quelling and meiotic silencing by unpaired DNA (MSUD) have been identified in the fungus Neurospora crassa, indicating that the majority of fungi possess the silencing machinery.

TL;DR: Interestingly, orthologous silencing proteins from different fungal clades are often clustered more closely than paralogous proteins in a fungus, indicating that duplication events occurred before speciation events.

TL;DR: Detailed protocols for measuring various PTI-associated phenotypes, including bacterial populations after pretreatment of leaves with PAMPs, induction of reporter genes, callose deposition, activation of mitogen-activated protein kinases, and a luciferase-based reporter system are provided.