Showing papers in "Fungal Genetics and Biology in 2005"

TL;DR: It is concluded that oligonucleotide barcode is a powerful tool for the routine identification of Hypocrea/Trichoderma species and should be useful as a complement to traditional methods.

TL;DR: Evidence is presented that the cell wall of mycelial species of the Ascomycotina is similarly organized and contains glycoproteins with comparable functions.

TL;DR: A pHANNIBAL-like silencing vector, pSilent-1, for ascomycete fungi, which carries a hygromycin resistance cassette and a transcriptional unit for hairpin RNA expression with a spacer of a cutinase gene intron from the rice blast fungus Magnaporthe oryzae, shows higher efficiency in silencing of the eGFP gene.

TL;DR: Despite high levels of variation in amino acid sequences, results of in silico prediction of protein secondary structure for Alt a 1 demonstrated a high degree of structural similarity for most of the species suggesting a conservation of function.

TL;DR: Genetic drift may be a driving force that is responsible for the loss of the entire aflatoxin gene cluster in nonaflatoxigenic A. flavus isolates when aflatoxins have lost their adaptive value in nature.

TL;DR: FM4-64 provides a useful framework for the phenotypic characterization of A. nidulans mutants affected in endocytosis and labels strongly the mitochondrial network and weakly the nuclear membrane.

TL;DR: A role of GintZnT1 in Zn compartmentalization and in the protection of G. intraradices against Zn stress is suggested.

TL;DR: There were differences in gene expression between hemolymph and cuticle and these mostly involved perception mechanisms, carbon metabolism, proteolysis, cell surface properties, and synthesis of toxic metabolites, which suggest previously unsuspected stratagems of fungal pathogenicity that can be tested experimentally.

TL;DR: It is shown that a gene cluster, including the F. graminearum PKS12 gene, is responsible for the biosynthesis of aurofusarin, and targeted mutagenesis of F. pseudograminearums showed an albino phenotype.

TL;DR: The tga1 gene deletion resulted in a complete loss of mycoparasitic overgrowth and lysis of Rhizoctonia solani, Botrytis cinerea, and Sclerotinia sclerotiorum during direct confrontation, although infection structure formation was unaffected.

TL;DR: An efficient method of gene replacement is developed based on Agrobacterium tumefaciens-mediated transformation with a mutant allele of the target gene flanked by the herpes simplex virus thymidine kinase gene as a conditional negative selection marker against ectopic transformants.

TL;DR: In this paper, the distribution of fungal mycelium in wood was locally resolved and semiquantitatively recorded using FTIR microscopy combined with a focal plane array detector and image analysis.

TL;DR: Northern blot and promoter sequence analyses support the implication of the protease P6281 in mycoparasitism and a novel aspartic protease (P6281) highly induced by fungal cell walls is identified.

TL;DR: In the ascomycete Gibberella zeae, the launch speed and kinetic energy of ascospores shot from perithecia are determined, and the source and magnitude of the pressure driving the launch are investigated, which supports the role of potassium ion flux in the mechanism.

TL;DR: The Deltahik1 strain showed increased sensitivity to high concentrations of sugars although its salt sensitivity was not elevated, suggesting that the rice blast fungus can distinguish osmostresses caused by high sugar concentrations and high salt concentrations.

TL;DR: Protection against fungal diseases could have been a powerful selective mechanism for endothermy in certain vertebrates.

TL;DR: Sequence analysis of the CYP51 gene from the progeny of a cross between DMI-sensitive and resistant Bgh isolates showed that both mutations segregate with resistance, which is consistent with CYP 51 controlling a major portion of DMI resistance.

TL;DR: Real-time RT-PCR revealed that expression of potato homologues of the amino acid biosynthesis genes increased during biotrophic and necrotrophic infection phases, and levels of free amino acids in the pre-infection stages were investigated.

TL;DR: The isolation and the characterisation of the LIP7 and LIP8 genes which were found to encode a 366 and a 371-amino acid precursor protein, respectively are described and it is suggested that no additional extracellular lipase remains to be discovered in Y. lipolytica.

TL;DR: A "Molecular Toolkit" comprising interchangeable promoters and marker genes to facilitate transformation of homobasidiomycete mushrooms is developed and it is demonstrated that a prerequisite for GFP expression in C. cinereus and A. bisporus is the presence of an intron.

TL;DR: Nine candidate fumonisin regulatory genes and a number of genes that may play a role in plant-fungal interaction were identified and evidence for a previously unidentified gene (FUM20) in the FUM gene cluster was provided.

TL;DR: The results suggest that the stimulating effect of enhanced organelle numbers may reflect an increase in the fluxes of penicillin and/or its precursors across the now much enlarged microbody membrane.

TL;DR: Measurements of chitinase activity from yeast cells of C. albicans showed that Cht2 is bound to cells, consistent with it being GPI-anchored while ChT3 is secreted into growth medium; Cht3 is also the principal, observed activity.

TL;DR: This is the first occasion when two enzymes of similar function and origin fall into phylogenetically distinct subfamilies within the expanding dendrogram of the class II fungal secretory heme peroxidases.

TL;DR: This work describes the adaptation of the Cre-loxP site-specific recombination system as a tool for the efficient and controlled disruption of C. albicans genes and generates plasmids containing the URA3, HIS1, and ARG4 markers for the reintegration of markers and target genes in control strains.

TL;DR: It is proposed that the specific effect of fructose on germination is not due to transport but rather to an as yet unknown intracellular sensing.

TL;DR: The results indicate that SirA contributes to self-protection against sirodesmin in L. maculans and suggest a transporter other than SirA is primarily responsible for efflux of endogenously produced siro Desmin.

TL;DR: To understand the effect of nitrogen source, carbon source, temperature, and pH on the regulation of AF biosynthesis, a targeted cDNA microarray consisting of genes associated with AF production over time was employed.

TL;DR: In this article, a family of 5 1,3α-d-glucan synthase-encoding genes in Aspergillus niger was identified and the predicted protein sequence indicated that the overall domain structure of 1, 3α-da-dglucans is conserved in fungi.

TL;DR: The differential effect of the various inhibitors suggests the fungal isoform of nitric oxide synthase (NOS) may be biochemically similar to mammalian constitutive NOS.