Hua-Fang Sheng

TL;DR: Millions of Illumina reads are determined for a comparison of bacterial communities in freshwater, intertidal wetland, and marine sediments along Pearl River, China, using a technically consistent approach to show that both taxon richness and evenness were the highest in freshwater sediment, medium in intert tidal sediment, and lowest in marine sediment.

TL;DR: To understand the generalizability of microbiota-based diagnostic models of metabolic disease, the gut microbiota was characterized of 7,009 individuals from 14 districts within 1 province in China and among phenotypes, host location showed the strongest associations with microbiota variations.

TL;DR: The bulk sediment inside the mature mangrove forest had the highest bacterial α-diversity, while the mudflat sediment without vegetation had the lowest, and the comparison of β-d diversity using principal component analysis and principal coordinate analysis with UniFrac metrics both showed that the spatial effects on bacterial communities were significant.

TL;DR: This study alerted even though FOS and GOS increased Bifidobacterium, they might have adverse effect on glucose metabolism by reducing butyrate-producing microbes.

TL;DR: A barcoded Illumina paired-end (PE) sequencing (BIPES) method that sequences each 16S V6 tag from both ends on the Illumina HiSeq 2000, and the PE reads are then overlapped to obtain the V 6 tag, and each of the BIPES reads costs less than 1/40 of a pyrosequencing read.