Compared with the single-marker detection scheme, the detection of multiple targets in the complex cell and biological environment can obtain more reliable detection results. Herein, we detected miRNA-21 and APE1 in two modes, AND and OR, respectively, based on gold nanoflares and simple logic components. In both modes, DNAzyme and APE1 can get rich fluorescence recovery results by breaking the DNA strands from the gold nanorods (AuNRs) and unquenching under different conditions. In vivo and in vitro experiments suggest that both nanoflares exhibit excellent biocompatibility and make efficient and sensitive judgments on the two targets. This strategy emphasizes the reuse nature of enzymes, and a small amount of target can generate a large amount of fluorescent signal in the logic device, which greatly reduces the detection limit when monitoring low-abundance targets. Since the short-stranded DNA component of the detection device is simple in composition and easy to program its probe sequence, it can be expanded into a detection system for the detection of other sets of related markers, which increases its potential for clinical application.

Smart Programmable Scalable Dual-Mode Diagnostic Logic Nanoflare Strategy for Dual-Tumor Marker Detection.

Organophosphorus chemical security from a peaceful perspective: sustainable practices in its synthesis, decontamination and detection

The invention relates to a preparation method of triphenyl phosphate. The preparation method comprises the following steps of: (1), adding a catalyst into molten phenol, putting a mixture into a reaction kettle, stirring the mixture until the temperature is reduced to be below 50 DEG C, stopping stirring and mixing phosphorus oxychloride; (2), performing constant pressure acid drainage and negative pressure acid drainage under a stirring condition to ensure that an acid value is less than or equal to 6mg KOH/g, and neutralizing and exhausting to obtain qualified coarse ester; and (3) raising the temperature of the qualified coarse ester to perform pressure reduction, distillation and separation and collecting a finished product; performing alkali washing and water washing, sampling and analyzing the finished product to ensure that the acid value is less than or equal to 0.05mg KOH/g, dehydrating and purifying, and sampling and analyzing the finished product to ensure that the acid value is less than or equal to 0.05mg KOH/g; and filtering to obtain triphenyl phosphate. The preparation method is easy to operate, mild in condition, short in reaction time, low in energy consumption, low in corrosion, low in pollution, and high in yield, and the product is pure, and the yield is up to more than 88 percent.

Preparation method of triphenyl phosphate

On-Water and Efficient Ullmann-Type O-Arylation Cross Coupling Reaction of Phenols and Aryl Tosylates in the Presence of Fe3O4@Starch-Au as Nanocatalyst

The invention discloses a preparation method of aluminium hypophosphite. The preparation method comprises the following steps of: a, preparing a sodium hypophosphite solution; b, preparing an aluminium nitrate solution; and c, uniformly mixing the sodium hypophosphite solution with the aluminium nitrate solution, regulating the pH (potential of Hydrogen) to 2-6, heating and reacting to form the target product aluminium hypophosphite. The method disclosed by the invention has the advantages that the reaction is carried out through directly taking water as solvent and the temperature is lower, so that the reaction is easy to control; the particle size of the obtained aluminium hypophosphite is less than 50 micrometers, the aluminium hypophosphite is white powder and no extra color processing is needed, so that the purity is higher and the yield of the obtained target product is 92-99%; the conventional synthetic process route is simplified and no toxic substance is produced, so that the preparation method is friendly to environment and low in cost; and high-efficiency fire retardant and combustion improver are obtained in the same reaction, so that the resource utilization rate is high and better industrial prospect is provided.

Preparation method of aluminium hypophosphite

The invention relates to a method for preparing aluminum hypophosphite. The method includes the following steps of firstly, preparing sodium hypophosphite solution with concentration of 2-6mol/L, adjusting pH (potential of hydrogen) to be 3-5 preferentially within 3-6.5; secondly, preparing aluminum sulfate solution; and thirdly, subjecting the sodium hypophosphite solution and the aluminum sulfate solution to reaction by heating on the acid condition to generate aluminum hypophosphite. The aluminum sulfate is added into the sodium hypophosphite solution for reaction within a certain temperature range, so that harms caused by volatilization of hypophosphorous acid during preparing of the aluminum hypophosphite are avoided, operation is simplified, yield of products is high, cost is low and industrialization is easy to realize.

Method for preparing aluminum hypophosphite

The invention discloses a preparation method for synthesizing phosphotriester under the catalysis of Lewis acid ionic liquid. According to the method, the phosphotriester is generated by performing ring-opening reaction of phosphorus oxychloride and an epoxide under the catalysis of a catalyst, wherein ionic liquid [A]-x[Lewis acid] formed by the catalyst is Lewis acid and A; and A is amine and a derivative thereof, imidazole or a derivative thereof or pyridine and a derivative thereof. According to the preparation method, the catalyst is low in cost of raw materials, high in catalytic efficiency, little in using amount and environment-friendly, and a process is simple; and the prepared phosphate flame retardant product is high in quality, small in acid value, good in color and high in purity.

Preparation method for synthesizing phosphotriester under catalysis of Lewis acid ionic liquid

G-quadruplex-based complexes have been widely used in various analytical methods due to their outstanding capabilities of generating colorimetric, fluorescent or electrochemical signals. However, since loop sequences in traditional G-quadruplex structures are quite short, it is difficult to establish biosensors solely using G-quadruplex-based complexes. Herein, we attempted to lengthen the loop sequences of G-quadruplex structures and found that G-quadruplex-hemin DNAzymes (G-DNAzymes) with long loops (even 30 nucleotides) maintain high peroxidase activity. In addition, the peroxidase activity is not affected by the hybridization of the long loop with its complementary counterpart. Consequently, G-DNAzyme can be endowed with an additional function by taking the long loop as a recognition element, which may facilitate the construction of diverse colorimetric biosensors. Furthermore, by designing an apurinic/apyrimidinic site or a complementary sequence of microRNA-21 (miRNA-21) in long loops, bifunctional G-DNAzymes can be split in the presence of apurinic/apyrimidinic endonuclease 1 (APE1) or miRNA-21, decreasing their peroxidase activities. Accordingly, APE1 and miRNA-21 are quantified using 3,3',5,5'-tetramethylbenzidine as a chromophore. Using the G-DNAzyme, APE1 can be detected in a linear range from 2.5 to 22.5 U mL-1 with a LOD of 1.8 U mL-1. It is to be noted that benefitting from duplex-specific nuclease-induced signal amplification, the linear range of the miRNA-21 biosensor is broadened to 5 orders of magnitude, while the limit of detection is as low as 73 fM. This work demonstrates that G-DNAzymes with long loops can both generate signals and recognize targets, providing an alternative strategy to design G-quadruplex-based analytical methods.

Huang Xiaodong

Papers

Method for preparing aluminum hypophosphite

Preparation method for synthesizing phosphotriester under catalysis of Lewis acid ionic liquid

Preparation method of aluminium hypophosphite

Fabrication of bifunctional G-quadruplex-hemin DNAzymes for colorimetric detection of apurinic/apyrimidinic endonuclease 1 and microRNA-21.

Green Synthesis of Triaryl Phosphates with POCl3 in Water