Showing papers by "Ian Chopra published in 1988"
TL;DR: The use of phenethyl alcohol (PEA) as a probe for signal sequence-dependent protein translocation in minicells was examined and it is predicted that PBP4 does not require a leader sequence for membrane insertion.
5 citations
5 citations
TL;DR: Bacterial penicillin-binding proteins were examined by two-dimensional electrophoresis to establish whether multiple isoelectric forms are widespread amongst PBPs, and the results are discussed in relation to current models of bacterial peptidoglycan structure.
Abstract: Some bacterial penicillin-binding proteins (PBPs) are reported to exist as multiple isoelectric forms. Other PBPs were examined by two-dimensional electrophoresis to establish whether multiple isoelectric forms are widespread amongst PBPs. Bacillus subtilis PBPs 3 and 5 and Escherichia coli PBPs 1b alpha and 1b gamma focussed as discrete spots with no evidence of multiple isoforms. However, E. coli PBPs 1b beta and 4 displayed isoelectric variants. The results are discussed in relation to current models of bacterial peptidoglycan structure.
1 citations