Showing papers by "Ichiro Ono published in 1995"
TL;DR: It is increased the likelihood that film or hydrocolloid dressings will be used more frequently in the future for treatment of burn wounds, ulcers or donor-site wounds since these dressings were shown to be more capable of retaining cytokines, particularly intrinsic growth factors secreted at the wound site.
103 citations
TL;DR: Results indicated that if recombinant human BMP is used clinically in the future, artificially fabricated HAP would be a suitable carrier.
Abstract: In this study, we investigated the efficacy of hydroxyapatite ceramic (HAP) as a carrier of bone morphogenetic protein (BMP) using porous HAP pellets artificially fabricated from limestone. After treatment with recombinant human BMP-2, the pellets were inserted beneath the cranial periosteum of rabbits, and the degree of osteogenesis was examined histologically. The degree of osteogenesis was also evaluated using image-analyzing procedures. Results showed that extensive bone formation had occurred around the pellets 3 weeks after insertion in the group that received pellets treated with recombinant human BMP alone as well as in the group that received recombinant human BMP in addition to type I collagen-treated HAP pellets. Subsequently, osteogenesis within the pellets slowly progressed over time, and by 9 weeks after insertion most of the pellet pores in both groups were filled with newly generated bone. The recombinant human BMP-collagen group, however, exhibited a significantly greater bone induction. These results indicated that if recombinant human BMP is used clinically in the future, artificially fabricated HAP would be a suitable carrier.
87 citations
TL;DR: The extent of bone induction, which was measured with a dual x-ray densitometer, that resulted from covering the hydroxyapatite pellet with periosteum alone (control group) was minimal and the usefulness of the technique was confirmed.
Abstract: Using a rabbit model, we evaluated the role of the periosteum in bone induction using hydroxyapatite ceramic pellets, some of which had been coated with bone morphogenetic protein. Eighteen rabbits were divided into two groups, a control group that received pellets soaked in phosphate-buffered saline alone and another group for which the pellets had been supplemented with bone morphogenetic protein. After making a skin incision on the head of each rabbit, two caudally pedicled periosteal flaps measuring 1 cm in width and 2.5 cm in length were elevated. These flaps were wrapped around hydroxyapatite pellets manufactured from limestone and fixed with sutures. After implantation, both groups of rabbits were returned to their cages, maintained for 3, 6, or 9 weeks [every group consisted of 3 rabbits (6 pellets)], and then sacrificed. In this study, the extent of bone induction, which was measured with a dual x-ray densitometer, that resulted from covering the hydroxyapatite pellet with periosteum alone (control group) was minimal. On the contrary, since osteogenesis from the periosteum toward the pores of the pellet was observed in the bone morphogenetic protein group much more than in the control group, the usefulness of our technique was confirmed. However, active osteogenesis was observed with subperiosteal implantation of the hydroxyapatite-bone morphogenetic protein complex in the bone morphogenetic protein group, but the osteogenesis observed was not distributed over the entire pellet.
44 citations
TL;DR: A new method for treating cryptotia using a rhomboid flap in the superior and anterior auricular regions to correct the contraction of skin in the helix in cases in which the deformity of the upper auricular portion is large and shortening in the anterior and posterior directions is significant and in which severe contraction of the helical skin is observed.
Abstract: Results following surgical treatment for cryptotia are as yet far from fully satisfactory in cases in which deformity of the upper auricular portion is severe and particularly when there is contraction of the helical skin and auricular cartilage. Therefore, improvements in operative procedures have been considered necessary. We have developed a new method for treating cryptotia using a rhomboid flap in the superior and anterior auricular regions to correct the contraction of skin in the helix in cases in which the deformity of the upper auricular portion is large and shortening in the anterior and posterior directions is significant and in which severe contraction of the helix is observed. In this report, we describe the operative procedures used and present the relatively favorable results obtained in three patients. Using these techniques, we have been able to elongate the contracted helix frequently observed in cases of cryptotia by preparing a flap in the anterior auricular region. This approach appears to be a useful and safe means of treating cryptotia and so-called constricted ear as well.
25 citations
TL;DR: It is likely that MR imaging has great potential as an auxiliary tool for the diagnosis of other types of malignant skin tumors, and the authors expect further technologic improvements in MR imaging in the future which will serve to increase the benefits obtained in its clinical applications.
24 citations
TL;DR: The results suggest that keratinocytes are a major source of cutaneous PDGF and that this factor may therefore play an important role in wound repair and in certain proliferative skin diseases.
Abstract: Platelet-derived growth factor (PDGF) is a potent mitogen for several mesenchymal cells and plays an important role in wound repair Three PDGF isoforms, PDGF-AA, PDGF-BB, and PDGF-AB, have been found to be generated in various tissues PDGF-AB production by normal human keratinocytes (NHKs), by human squamous cell carcinoma cell line (HSC-1) cells, and by human dermal fibroblasts (HDFs) was studied in the presence of agents which influence cell growth Both NHKs and HSC-1 cells spontaneously produced and secreted PDGF-AB NHKs grown in keratinocyte growth medium produced more PDGF-AB than did those grown in keratinocyte basic medium Phorbol 12-myristate 13-acetate inhibited PDGF-AB production in NHKs but promoted its production in HSC-1 cells 1,25-dihydroxyvitamin D3 up-regulated PDGF-AB production, whereas etretinate did not High levels of calcium in the culture medium induced little change in cellular PDGF-AB levels Prostaglandin E1 slightly inhibited PDGF-AB production, transforming growth factor beta 1 promoted PDGF-AB production and interferon-gamma, interleukin-1 alpha, and tumor necrosis factor-alpha failed to exert any influence at all Cultured HDFs did not produce any detectable PDGF-AB These results suggest that keratinocytes are a major source of cutaneous PDGF and that this factor may therefore play an important role in wound repair and in certain proliferative skin diseases
23 citations
TL;DR: It is suggested that IFN‐γ has different regulatory effects on IL‐6 and IL‐8 secretion in NHKs and HDFs, depending on the stimulus.
Abstract: Gamma-interferon (IFN-gamma) is produced by T cells and plays an important role in immunological and inflammatory processes. To determine the effects of IFN-gamma on interleukin (IL)-6 and IL-8 secretion, normal human keratinocytes (NHKs), human squamous cell carcinoma cell line (HSC-1) cells, and human dermal fibroblasts (HDFs) were incubated with 100 U/ml of recombinant (r) IFN-gamma in the presence of various stimulants. HSC-1 cells and HDFs spontaneously secreted both IL-6 and IL-8 into the culture medium. NHKs secreted detectable levels of IL-8, but not of IL-6, and IL-8 secretion increased over 20 fold by stimulation with 10 nM of phorbol 12-myristate 13-acetate (PMA). rIFN-gamma inhibited IL-8 secretion in both HSC-1 cells and PMA-stimulated NHKs. On the other hand, it enhanced IL-1 alpha- and TNF alpha-induced IL-8 secretion in NHKs. In HDFs, rIFN-gamma inhibited IL-8 secretion, but enhanced secretion of IL-6, regardless of whether they were stimulated with IL-1 alpha or PMA. These results suggest that IFN-gamma has different regulatory effects on IL-6 and IL-8 secretion in NHKs and HDFs, depending on the stimulus.
23 citations
TL;DR: The newly synthesized prostaglandin (PG) I1 analogues were compared with PGE1 in terms of their biological effects on cultured normal human keratinocytes and human dermal fibroblasts in order to evaluate their therapeutic potential for cutaneous wound healing.
Abstract: The newly synthesized prostaglandin (PG) I1 analogues, SM-10902 and SM-10906, were compared with PGE1 in terms of their biological effects on cultured normal human keratinocytes (NHKs) and human dermal fibroblasts (HDFs) in order to evaluate their therapeutic potential for cutaneous wound healing. The PGI1 analogues had a direct effect on cell proliferation of HDFs as did PGE1, but inibited cell growth of NHKs in contrast to the stimulatory effect observed with PGE1. In contrast to NHKs stimulated with PGI1 analogues, which exhibited low levels of adenosine 3,5-cyclic monophosphate (cAMP). HDFs stimulated with these analogues responded in a dose-dependent manner with extremely high levels of cAMP. Conditioned media (CM) derived from media in which HDFs had been incubated with both the PGI1 analogues promoted NHK proliferation. HDF production of interleukin (IL)-6 increased in response to the PGI1 analogues. Since IL-6 was shown to promote cell growth of NHKs, enhancement of NHK proliferation by CM was thought to be due to IL-6 derived from HDFs stimulated with the PGI1 analogues.
20 citations
TL;DR: Antihuman IFN-gamma antibody inhibited IL-1 alpha release by HSC-1 cells stimulated with rIFN-Gamma, suggesting that IL- 1 alpha release from keratinocytes is upregulated by IFN -gamma.
Abstract: It is known that keratinocytes produce and secrete interleukin-1 (IL-1) de novo and that this process can be enhanced by various stimulators. IL-1 has been shown to be a potent proin-flammatory cytoki
16 citations
31 Oct 1995
2 citations
TL;DR: Using a temporoparietal fascial flap and hydroxyapatite ceramics, a patient for complications after a neurosurgical operation for glossopharyngeal neuralgia was treated for complications resulting from fistulas of mastoid air cells associated with a subcutaneous dead space.
Abstract: Using a temporoparietal fascial flap and hydroxyapatite ceramics, we treated a patient for complications after a neurosurgical operation for glossopharyngeal neuralgia. These consisted of pneumocephalus and cerebrospinal fluid rhinorrhea resulting from fistulas of mastoid air cells associated with a subcutaneous dead space. By means of the temporoparietal fascial flap, we were able to fill the dead space and reinforce the repaired dural and mastoid lesion as well. Hydroxyapatite ceramics were also useful for closing the mastoid air cell fistulas.