Imen Lassadi

TL;DR: It is found that all DSBs trigger large γH2AX domains, which spread out from the DSB in a bidirectional, discontinuous and not necessarily symmetrical manner, which is the first genome‐wide mapping of γh2AX around D SBs, indicating that mechanisms may exist to protect gene transcription from γ H2AX spreading and from the chromatin rearrangements induced by DSBS.

TL;DR: A novel Lab-on-Chip technology for3D particle tracking in living cells based on V-shaped micro-mirrors, which are used to observe fluorescent specimens from multiple vantage points, providing simultaneous stereo-images that can be recombined for 3D reconstruction.

TL;DR: Deletion of a 1-kb subregion within the RE, which is not necessary during switching, abolished mating-type-dependent chromosome folding and created a composite element with one subregion essential for donor selection during switching and a separate region involved in modulating chromosome conformation.

TL;DR: A method to determine chromosome conformation from relative positions of three fluorescently tagged DNA in living cells imaged in 3D is developed and is applicable to identify and characterize dynamic chromosome conformations in any cell type.

TL;DR: Detailed protocols for FROS usage and analysis in the yeast Saccharomyces cerevisiae are described, which facilitate the tagging of up to three different loci simultaneously.