https://iopscience.iop.org/article/10.1088/2516-1091/ab23df/pdf

The emergence of 3D bioprinting in organ-on-chip systems

The invention relates to culturing brain endothelial cells, and optionally astrocytes and neurons in a fluidic device under conditions whereby the cells mimic the structure and function of the blood brain barrier. Culture of such cells in a microfluidic device, whether alone or in combination with other cells, drives maturation and/or differentiation further than existing systems.

Development of spinal cord on a microfluidic chip

The present invention relates to the use of gels for cell cultures, including but not limited to microfluidic devices and transwell devices, for culturing cells, such as organ cells, e.g. airway cells, intestinal cells, etc., and co-culturing cells, (e.g. parenchymal cells and endothelial cells, etc). As one example, the use of gels results in improved lung cell cultures, such as when using transwells and microfluidic devices, (e.g. for culturing healthy airway epithelial cells, culturing diseased airway epithelial cells, e.g., CF epithelial cells that are ciliated).

Compositions and methods of using partial gel layers in a microfluidic device

The invention generally relates to a microfluidic platforms or “chips” for testing and conducting experiments on the International Space Station (ISS). More specifically, microfluidic Brain-On-Chip, comprising neuronal and vascular endothelial cells, will be analyzed in both healthy and inflamed states to assess how the circumstances of space travel affect the human brain.

Effects of space travel on human brain cells

This application relates to a method for identifying a drug candidate capable of increasing or decreasing barrier tissue integrity of endothelial cells. Moreover, this application relates to the use of a tight junction gene transcriptional reporter as a surrogate marker of transendothelial barrier integrity.

Methods for assessing transendothelial barrier integrity

Microfluidic Model Of The Blood Brain Barrier

The invention relates to culturing motor neuron cells together with skeletal muscle cells in a fluidic device under conditions whereby the interaction of these cells mimic the structure and function of the neuromuscular junction (NMJ) providing a NMJ-on-chip. Good viability, formation of myo-fibers and function of skeletal muscle cells on fluidic chips allow for measurements of muscle cell contractions. Embodiments of motor neurons co-cultures with contractile myo-fibers are contemplated for use with modeling diseases affecting NMJ's, e.g. Amyotrophic lateral sclerosis (ALS).

Neuromuscular Junction: NMJ-ON-CHIP

Compositions, devices and methods are described for improving adhesion, attachment, and/or differentiation of cells in a microfluidic device or chip. In one embodiment, one or more ECM proteins are covalently coupled to the surface of a microchannel of a microfluidic device. The microfluidic devices can be stored or used immediately for culture and/or support of living cells such as mammalian cells, and/or for simulating a function of a tissue, e.g., a liver tissue, muscle tissue, etc. Extended adhesion and viability with sustained function over time is observed.

Compositions and methods of cell attachment

L'invention concerne la culture de cellules endotheliales cerebrales, et eventuellement d'astrocytes et de neurones dans un dispositif fluidique dans des conditions dans lesquelles les cellules imitent la structure et la fonction de la barriere hemato-encephalique. La culture de telles cellules dans un dispositif microfluidique, soit seules soit en combinaison avec d'autres cellules, entraine une maturation et/ou une differenciation plus poussee que dans les systemes existants.

Jacob Fraser

Papers

Microfluidic Model Of The Blood Brain Barrier

Development of spinal cord on a microfluidic chip

Neuromuscular Junction: NMJ-ON-CHIP

Compositions and methods of cell attachment

Modèle microfluidique de la barrière hémato-encéphalique