Showing papers by "James D. Young published in 1984"

TL;DR: Exposure of site-bound [3H]nitrobenzylthioinosine to high intensity U.V. light resulted in the photoaffinity labelling of lung proteins with apparent molecular weights similar to that of the human erythrocyte nucleoside transporter (45,000-65,000).

TL;DR: The results suggest that the nitrobenzylthioinosine-binding site (s) and the permeation site(s) of the transporter are present on the same transporter element.

TL;DR: Plasma membrane-enriched fractions from disrupted S49 lymphoma cells contained high affinity sites for [3H]nitrobenzylthioinosine, a potent and specific inhibitor of nucleoside transport, which resulted in covalent radiolabeling of a membrane protein which migrated on sodium dodecyl sulfate-polyacrylamide gels.

TL;DR: This study showed that [3H]NBMPR molecules reversibly bound to intact S49 and L5178Y mouse lymphoma cells became covalently bound upon exposure to UV light.

TL;DR: Guinea pig reticulocytes are shown to possess an Na+-dependent glycine transporter which also requires Cl- for activity, and the properties of this carrier closely resemble those of System Gly previously demonstrated in pigeon and human erythrocytes.