James P. Freyer

TL;DR: 3-D in vitro systems for drug development, with a focus on screening for novel antitumor drugs, are addressed, and the advantages and limitations of these systems of intermediate complexity are discussed.

TL;DR: Optical diagnostics are expected to be sensitive to organelle morphology but not directly to the size and shape of the cells, as measured of isolated organelles indicate.

TL;DR: A new mathematical model for avascular tumor growth and development that spans three distinct scales is presented and predicts the microenvironmental conditions required for tumor cell survival and accurately predicts spheroid growth curves under different external nutrient supply conditions.

TL;DR: A model is presented to explain the observed spheroid growth characteristics by proposing a competition between externally supplied growth and viability-promoting factors and internally generated inhibitory factors produced by the process of necrosis, which has critical implications for the use of spheroids as models of cellular growth in tumors.

TL;DR: Raman spectra of cells and nuclei from cultures in the plateau (nonproliferating) and exponential phases of growth were measured and show that Raman spectroscopy can monitor changes due to cell proliferation, which will be important for Raman detection of rapidly dividing populations of cancer cells in vivo.