J
Jay M. Short
Researcher at Stratagene
Publications - 71
Citations - 14209
Jay M. Short is an academic researcher from Stratagene. The author has contributed to research in topics: Gene & Mutant. The author has an hindex of 41, co-authored 66 publications receiving 13812 citations. Previous affiliations of Jay M. Short include Case Western Reserve University & Torrey Pines Institute for Molecular Studies.
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Patent
High throughput screening for novel enzymes
Jay M. Short,Martin Keller +1 more
TL;DR: In this article, a process for identifying clones having a specified activity of interest by generating one or more expression libraries derived from nucleic acid directly or indirectly isolated from the environment, exposing said libraries to a particular substrate or substrate of interest, and screening said exposed libraries utilizing a fluorescence activated cell sorter to identify clones which react with the substrate or substrates.
Journal ArticleDOI
Identification of human antibody fragment clones specific for tetanus toxoid in a bacteriophage lambda immunoexpression library.
R L Mullinax,E A Gross,J R Amberg,B N Hay,H H Hogrefe,M M Kubitz,A Greener,M Alting-Mees,D Ardourel,Jay M. Short +9 more
TL;DR: A molecular biology approach to the identification of human monoclonal antibodies was applied and it is estimated that this human immunoexpression library contains 20,000 clones with high affinity and specificity to the authors' chosen antigen.
Patent
Production and use of normalized dna libraries
Jay M. Short,Eric J. Mathur +1 more
TL;DR: In this paper, a process for forming a normalized genomic DNA library from an environmental sample by isolating a genomic DNA population from the environmental sample, analyzing the complexity of the genomic DNA populations so isolated, amplifying the copy number of the DNA population so isolated and recovering a fraction of the isolated genomic DNA having a desired characteristic is described.
Patent
Directed evolution of thermophilic enzymes
TL;DR: In this article, a mutagenized version of the wild-type thermostable enzyme was used to produce a thermophilic enzyme which was stable at a temperature of 50°C or lower.
Journal ArticleDOI
Identification of a cAMP regulatory region in the gene for rat cytosolic phosphoenolpyruvate carboxykinase (GTP). Use of chimeric genes transfected into hepatoma cells.
TL;DR: There was no relationship between the number of copies of the PEPCK-TK gene integrated in the various cell lines and either the basal level of TK activity or its inducibility of Bt2cAMP.