J
Jay M. Short
Researcher at Stratagene
Publications - 71
Citations - 14209
Jay M. Short is an academic researcher from Stratagene. The author has contributed to research in topics: Gene & Mutant. The author has an hindex of 41, co-authored 66 publications receiving 13812 citations. Previous affiliations of Jay M. Short include Case Western Reserve University & Torrey Pines Institute for Molecular Studies.
Papers
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Journal ArticleDOI
Cloning of the immunological repertoire in Escherichia coli for generation of monoclonal catalytic antibodies: construction of a heavy chain variable region-specific cDNA library.
Lakshmi Sastry,Michelle A. Alting-Mees,W.D. Huse,Jay M. Short,J.A. Sorge,B N Hay,Kim D. Janda,Stephen J. Benkovic,Richard A. Lerner +8 more
TL;DR: This work presents a method for the establishment of a highly diverse heavy chain variable region library and may provide an alternative to the hybridoma methodology for accessing the monoclonal antibody specificity of the immune system.
Patent
Method of DNA reassembly by interrupting synthesis
TL;DR: In this paper, the authors describe a process of performing sexual PCR which includes generating random polynucleotides by interrupting or blocking a synthesis or amplification process to show or halt synthesis of at least one polynotide, optionally amplifying the polynuclotide, and reannealing the poynucleotide to produce random mutant polypeptides.
Journal ArticleDOI
Exploring nitrilase sequence space for enantioselective catalysis.
Dan E. Robertson,Jennifer Ann Chaplin,Grace Desantis,Mircea Podar,Mark Madden,Ellen Chi,Toby Richardson,Aileen Milan,Mark E. Miller,David P. Weiner,Kelvin K. L. Wong,Jeffrey B. McQuaid,Bob Farwell,Lori A. Preston,Xuqiu Tan,Marjory A. Snead,Martin Keller,Eric J. Mathur,Patricia L. Kretz,Mark J. Burk,Jay M. Short +20 more
TL;DR: 137 unique nitrilases, discovered from screening of >600 biotope-specific environmental DNA (eDNA) libraries, were characterized, providing a catalytic toolbox for enantiospecific synthesis of a variety of carboxylic acid derivatives, as well as an intriguing library for evolutionary and structural analyses.
Journal ArticleDOI
A Novel, High Performance Enzyme for Starch Liquefaction: DISCOVERY AND OPTIMIZATION OF A LOW pH, THERMOSTABLE α-AMYLASE *
Toby Richardson,Xuqiu Tan,Gerhard Frey,Walter Callen,Mark Cabell,David Lam,John L. Macomber,Jay M. Short,Dan E. Robertson,Carl Miller +9 more
TL;DR: The synergistic approach of combining environmental discovery and laboratory evolution for identification and optimization of industrially important biocatalysts with exceptional process compatibility and economics is described.