J
Jessica I. Spitzer
Researcher at Howard Hughes Medical Institute
Publications - 4
Citations - 1041
Jessica I. Spitzer is an academic researcher from Howard Hughes Medical Institute. The author has contributed to research in topics: Promoter & Nuclease protection assay. The author has an hindex of 4, co-authored 4 publications receiving 978 citations.
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Journal ArticleDOI
miRNAs in human cancer.
TL;DR: A critical overview of miRNA dysregulation in cancer is provided, first discussing the methods currently available for studying the role of miRNAs in cancer and then reviewing miRNA genomic organization, biogenesis and mechanism of target recognition.
Book ChapterDOI
PAR-CLIP (Photoactivatable Ribonucleoside-Enhanced Crosslinking and Immunoprecipitation): a step-by-step protocol to the transcriptome-wide identification of binding sites of RNA-binding proteins.
Jessica I. Spitzer,Markus Hafner,Markus Landthaler,Manuel Ascano,Thalia A. Farazi,Greg S. Wardle,Jeff Nusbaum,Mohsen Khorshid,Lukas Burger,Mihaela Zavolan,Thomas Tuschl +10 more
TL;DR: By introducing photoreactive nucleosides that generate characteristic sequence changes upon crosslinking, this protocol allows one to separate RNA segments bound by the protein of interest from the background un-crosslinked RNAs.
Book ChapterDOI
Rapid creation of stable mammalian cell lines for regulated expression of proteins using the Gateway® recombination cloning technology and Flp-In T-REx® lines.
TL;DR: The aim is to determine the mRNA targets of the hundreds of RNA-binding proteins encoded in the human genome by the isolation and molecular characterization of their ribonucleoprotein complexes (RNPs).
Journal ArticleDOI
mRNA and Protein Levels of FUS, EWSR1 and TAF15 are Upregulated in Liposarcoma
Jessica I. Spitzer,Stacy Ugras,Simon Runge,Penelope DeCarolis,C. R. Antonescu,Tom Tuschl,Samuel Singer +6 more
TL;DR: The results suggest that the powerful promoters of FET genes are predominantly responsible for the oncogenic effect of transcription factor translocations in sarcomas.