Showing papers by "Jimmy K. Eng published in 1996"
TL;DR: Searching protein and nucleotide databases with MS data allows accurate identification of amino acid sequences and helps clarify the role of residues in amino acid chains.
Abstract: Searching protein and nucleotide databases with MS data allows accurate identification of amino acid sequences
79 citations
TL;DR: Given the need to relate mounting genome sequence information with corresponding suites of proteins that comprise the cellular molecular machinery, tandem mass spectrometry appears destined to play the leading role in accelerating protein identification on the large scale required.
78 citations
TL;DR: An overview of methods for the study of complex biological processes by using micro-column liquid chromatography-electrospray ionization tandem mass spectrometry is provided in this article.
Abstract: An overview is provided of methods for the study of complex biological processes by using micro-column liquid chromatography-electrospray ionization tandem mass spectrometry. Procedures discussed include electrospray ionization, micro-column liquid chromatography, tandem mass spectrometry, tandem mass spectra data interpretation for peptides, and database searching with mass spectral data. Several problems in immunology are discussed to illustrate this approach.
74 citations
Journal Article•
TL;DR: The feasibility of typing single nucleotide polymorphisms (SNPs) by using a semiautomated method for analyzing human DNA samples is explored and a panel of 20 markers has been developed for DNA typing and has been tested using a sample panel from the CEPH pedigrees.
Abstract: The use of DNA typing in human genome analysis is increasing and finding widespread application in the area of forensic and paternity testing. In this report, we explore the feasibility of typing single nucleotide polymorphisms (SNPs) by using a semiautomated method for analyzing human DNA samples. In this approach, PCR is used to amplify segments of human DNA containing a common SNP. Allelic nucleotides in the amplified product are then typed by a colorimetric implementation of the oligonucleotide ligation assay (OLA). The results of the combined assay, PCR/OLA, are read directly by a spectrophotometer; the absorbances are compiled; and the genotypes are automatically determined. A panel of 20 markers has been developed for DNA typing and has been tested using a sample panel from the CEPH pedigrees (CEPH parents). The results of this typing, as well as the potential to apply this method to larger populations, are discussed.
61 citations
Journal Article•
2 citations
05 May 1996
2 citations
01 Apr 1996
TL;DR: Preliminary data is shown for extending the cross-correlation database searching method to multi-stage mass spectra of polypeptides to provide a means to directly identify large polypePTides or proteins without the necessity of proteolytic hydrolysis to produce peptides amenable to tandem mass spectrometry.
Abstract: Database searching with tandem mass spectra has been demonstrated as a powerful method to correlate peptides and proteins to gene sequences for identification. In this study preliminary data is shown for extending the cross-correlation database searching method to multi-stage mass spectra of polypeptides. This method may provide a means to directly identify large polypeptides or proteins without the necessity of proteolytic hydrolysis to produce peptides amenable to tandem mass spectrometry.