J
John R. Yates
Researcher at Scripps Research Institute
Publications - 1074
Citations - 139869
John R. Yates is an academic researcher from Scripps Research Institute. The author has contributed to research in topics: Proteomics & Proteome. The author has an hindex of 177, co-authored 1036 publications receiving 129029 citations. Previous affiliations of John R. Yates include Salk Institute for Biological Studies & University of California, San Diego.
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An approach to correlate tandem mass spectral data of peptides with amino acid sequences in a protein database.
TL;DR: The approach described in this manuscript provides a convenient method to interpret tandem mass spectra with known sequences in a protein database.
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Large-scale analysis of the yeast proteome by multidimensional protein identification technology.
TL;DR: MudPIT was applied to the proteome of the Saccharomyces cerevisiae strain BJ5460 grown to mid-log phase and yielded the largest proteome analysis to date, identifying 131 proteins with three or more predicted transmembrane domains which allowed us to map the soluble domains of many of the integral membrane proteins.
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A Model for Random Sampling and Estimation of Relative Protein Abundance in Shotgun Proteomics
TL;DR: A linear dynamic range over 2 orders of magnitude is demonstrated by using the number of spectra (spectral sampling) acquired for each protein by the data-dependent acquisition of peptides eluting into the mass spectrometer.
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Wnt proteins are lipid-modified and can act as stem cell growth factors
Karl Willert,Jeffrey Brown,Esther Danenberg,Andrew W. Duncan,Irving L. Weissman,Tannishtha Reya,John R. Yates,Roel Nusse +7 more
TL;DR: This work isolated active Wnt molecules, including the product of the mouse Wnt3a gene, and found the proteins to be palmitoylated on a conserved cysteine, indicating that the lipid is important for signalling.
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Direct analysis of protein complexes using mass spectrometry
Andrew J. Link,Jimmy K. Eng,David Schieltz,Edwin Carmack,Gregory J. Mize,David R. Morris,Barbara M. Garvik,John R. Yates +7 more
TL;DR: A rapid, sensitive process for comprehensively identifying proteins in macromolecular complexes that uses multidimensional liquid chromatography and tandem mass spectrometry to separate and fragment peptides is described.