J
Jingxuan Cui
Researcher at Oak Ridge National Laboratory
Publications - 9
Citations - 188
Jingxuan Cui is an academic researcher from Oak Ridge National Laboratory. The author has contributed to research in topics: Clostridium thermocellum & Pyruvate, phosphate dikinase. The author has an hindex of 5, co-authored 9 publications receiving 128 citations. Previous affiliations of Jingxuan Cui include Dartmouth College.
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Journal ArticleDOI
Metabolic engineering of Clostridium thermocellum for n -butanol production from cellulose
Liang Tian,Liang Tian,Peter M. Conway,Nicholas D. Cervenka,Jingxuan Cui,Jingxuan Cui,Marybeth Maloney,Marybeth Maloney,Daniel G. Olson,Daniel G. Olson,Lee R. Lynd,Lee R. Lynd +11 more
TL;DR: E engineering C. thermocellum to produce n-butanol by engineering the protein sequence of key enzymes in the pathway, which increased the n- butanol titer by 2.2-fold and increased n- Butanol production by adding ethanol to the growth media.
Journal ArticleDOI
Glycolysis without pyruvate kinase in Clostridium thermocellum.
Daniel G. Olson,Daniel G. Olson,Manuel Hörl,Tobias Fuhrer,Jingxuan Cui,Jilai Zhou,Marybeth Maloney,Daniel Amador-Noguez,Liang Tian,Uwe Sauer,Lee R. Lynd,Lee R. Lynd +11 more
TL;DR: Deletion of the ppdk gene resulted in a redirection of all pyruvate flux through the malate shunt, providing the first direct evidence of the in-vivo function of the malates shunt.
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Expressing the Thermoanaerobacterium saccharolyticum pforA in engineered Clostridium thermocellum improves ethanol production.
Shuen Hon,Shuen Hon,Evert K. Holwerda,Evert K. Holwerda,Robert S. Worthen,Robert S. Worthen,Marybeth Maloney,Marybeth Maloney,Liang Tian,Liang Tian,Jingxuan Cui,Jingxuan Cui,Paul P. Lin,Paul P. Lin,Lee R. Lynd,Daniel G. Olson,Daniel G. Olson +16 more
TL;DR: It is demonstrated that the pforA gene can improve ethanol production in C. thermocellum as part of the T. saccharolyticum pyruvate-to-ethanol pathway.
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Characterization of the Clostridium thermocellum AdhE, NfnAB, ferredoxin and Pfor proteins for their ability to support high titer ethanol production in Thermoanaerobacterium saccharolyticum
TL;DR: The thermophilic anaerobes Thermoanaerobacterium saccharolyticum and Clostridium thermocellum are good candidates for lignocellulosic ethanol production and it is demonstrated that the reason for reduced ethanol production is that the Ct_pfor1 is inhibited by accumulation of ethanol and NADH, while Ts_p forA is not.
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The pentose phosphate pathway of cellulolytic clostridia relies on 6-phosphofructokinase instead of transaldolase
Jeroen Girwar Koendjbiharie,Shuen Hon,Shuen Hon,Martin Pabst,Robert Hooftman,David M. Stevenson,Jingxuan Cui,Jingxuan Cui,Daniel Amador-Noguez,Daniel Amador-Noguez,Lee R. Lynd,Lee R. Lynd,Daniel G. Olson,Daniel G. Olson,Richard van Kranenburg +14 more
TL;DR: The results confirm the existence of an SBP pathway for pentose assimilation in cellulolytic clostridia and demonstrate that a second PFK of C. thermosuccinogenes that operates with ATP and GTP exhibits unusual kinetics toward F6P, as it appears to have an extremely high degree of cooperative binding, resulting in a virtual on/off switch for substrate concentrations near its K½ value.