Showing papers in "Metabolic Engineering in 2017"

TL;DR: An efficient biosynthetic pathway capable of producing 100-fold more β-carotene than the baseline construct was generated using strong promoters and multiple gene copies for each of the 12 steps.

TL;DR: It is demonstrated that AOR is critical to ethanol formation in acetogens and inactivation of AdhE led to consistently enhanced autotrophic ethanol production (up to 180%).

TL;DR: A rapid genome engineering strategy to scarlessly knock out one or more genes in C. glutamicum in sequential and iterative manner is reported to expedite metabolic engineering of the microorganism.

TL;DR: By incorporating the pathway to synthesize the flavanone naringenin, the first example of in vivo conversion of methanol into a specialty chemical in E. coli is demonstrated, demonstrating a cycling RuMP pathway for meethanol assimilation to support growth.

TL;DR: This review summarizes the enabling technologies for biological foundries or biofoundries as well as their early successes and remaining challenges.

TL;DR: The power of plant transient transfection technology for rapid, scalable biosynthesis and isolation of triterpenes, one of the largest and most structurally diverse families of plant natural products, is demonstrated and has the potential to reinvigorate drug discovery pipelines.

TL;DR: A novel regulatory target, glucose-6-phosphate dehydrogenase (G6PD) from the pentose phosphate pathway (PPP), is presented in boosting microalgal lipid accumulation by enhancing NADPH supply and demonstrate that G6PD is a promising target for metabolic engineering.

TL;DR: This research sets a good reference for desired compounds overproduction in microorganisms in terms of manipulation of key enzymes by protein engineering and metabolic engineering and opens large opportunities for other microbial production of monoterpenes.

TL;DR: This report demonstrates that pathway engineering in yeast through transforming the subcellular morphology rather than altering metabolic fluxes is a powerful strategy to increase yields of bioactive plant-derived products in heterologous hosts.

TL;DR: Production using the tolerant strains resulted in 37g/L of L-serine with a 24% mass yield, similar to the highest production reported for any organism thereby highlighting the potential of ALE for industrial biotechnology.

TL;DR: It is identified for the first time that acetic acid overflow is caused by CoA-transfer reaction from acetyl-CoA to succinate in mitochondria rather than pyruvate decarboxylation reaction in SDH negative Y. lipolytica, and an efficient succinic acid production strain with great industrial prospects is reported.

TL;DR: Quantitative analysis of co-factor balances (NADH/FADH2, NADPH, and ATP) for different growth conditions provided new insights regarding the interplay of energy and redox metabolism and the impact on E. coli cell physiology.

TL;DR: This work has demonstrated high-level production of ginsenoside Rh2 in Saccharomyces cerevisiae via repurposing an inherently promiscuous glycosyltransferase, UGT51, through fermentation in yeast through fed-batch fermentation in a 5-L bioreactor.

TL;DR: The developed twin-clostridial consortium serves as a promising platform for ABE fermentation from lignocellulose by CBP and approximates to that achieved from a starchy feedstock.

TL;DR: The engineered strain was modified to incorporate the pentose transport gene araE and xylose catabolic gene xylAB, allowing for the simultaneous utilization of glucose andxylose, opening the way toward 3-HP production from abundant lignocellulosic feedstocks.

TL;DR: The discovery of a new enzyme (Eat1) from the yeast Wickerhamomyces anomalus that resulted in high ethyl acetate production when expressed in Saccharomyces cerevisiae and Escherichia coli is described.

TL;DR: This work provides a platform that enables modular construction, optimization and characterization to facilitate the development of FL-producing cell factories and promotes the efficient biosynthesis of 2'-FL and 3-FL in engineered Escherichia coli.

TL;DR: Improved titer and yield of fatty alcohols are improved using an approach involving quantitative analysis of protein levels and metabolic flux, engineering enzyme level and localization, pull-push-block engineering of carbon flux, and cofactor balancing.

TL;DR: The results demonstrate that the combined optimization of de novo fatty acid biosynthesis, storage lipid assembly and lipid turnover in leaf tissue results in a major overhaul of the plant central carbon allocation and lipid metabolism.

TL;DR: The feasibility of improving key enzyme activities in important biosynthetic pathways with the aid of designed biosensors of pathway products is demonstrated.

TL;DR: An improved synthetic photoautotroph/chemoheterotroph consortial design in which sucrose secreted by S. elongatus CscB directly supports the bacterium Halomonas boliviensis, a natural producer of the bioplastic precursor, PHB, achieves PHB productivities that match or exceed those of traditionally engineered cyanobacterial monocultures.

TL;DR: GEMs have great potential for translational research on cancer and will therefore become of increasing importance in the future, as they allow quantitative flux predictions using flux balance analysis (FBA).

TL;DR: De novo production of phloretin, the first committed dihydrochalcone, was achieved by co-expression of additional relevant pathway enzymes by exploiting substrate flexibility of enzymes involved in flavonoid biosynthesis.

TL;DR: This work establishes a rapid method for fine-tuned, graded expression of pathway enzymes via dCas9 regulation by varying sgRNA target location as the dominant parameter and envision that this technique can be used to complement genome-scale metabolic models by experimentally mapping the flux sensitivity of the entire genome to desired phenotypes.

TL;DR: Several novel phage-derived expression systems used for transcriptional control in non- model bacteria are reported and envision these T7-like expression systems to benefit metabolic engineering in other non-model organisms.

TL;DR: This work demonstrated the promising potential of microbial co-cultures for prevention of side-reactions and the first report about microbial production of caffeyl alcohol and coniferyl alcohol.

TL;DR: It is demonstrated that an engineered ERAD mechanism can be used to balance flux competition between the endogenous sterol pathway and an introduced bio-product pathways at the FPP node and the approach of protein degradation in general might be more widely applied to improve metabolic engineering outcomes.

TL;DR: A genome-scale metabolic model (GEM) is used to explore alternative ATP-generating pathways in the gas-fermenting acetogen Clostridium autoethanogenum to offer a potential route for supplying cells with ATP, while demonstrating the usefulness of metabolic modelling for the discovery of native pathways for stimulating growth or enhancing energy availability.

TL;DR: The design principles of constructing a transcriptional factor-based malonyl-CoA sensor with superior detection limit, high sensitivity and broad dynamic range are reviewed and it is envisioned that the genetically-encoded malony l-coA sensor will be an indispensable tool to optimize cell metabolism and cost-competitively manufacture maloneyl- CoA-derived compounds.

TL;DR: The membrane engineering strategy developed in this work opens up a new direction for engineering and improving microbial terpene producers and it is quite possible that a wide range of strains used to produce hydrophobic compounds can be further improved using this novel engineering strategy.