Learning Deconvolution Network for Semantic Segmentation

2004아테네 올림픽대회출전 한국레슬링 대표선수의 무산소성 운동능력 및 등속성 근력

Here we describe a method for fabricating a primary human Small Intestine-on-a-Chip (Intestine Chip) containing epithelial cells isolated from healthy regions of intestinal biopsies. The primary epithelial cells are expanded as 3D organoids, dissociated, and cultured on a porous membrane within a microfluidic device with human intestinal microvascular endothelium cultured in a parallel microchannel under flow and cyclic deformation. In the Intestine Chip, the epithelium forms villi-like projections lined by polarized epithelial cells that undergo multi-lineage differentiation similar to that of intestinal organoids, however, these cells expose their apical surfaces to an open lumen and interface with endothelium. Transcriptomic analysis also indicates that the Intestine Chip more closely mimics whole human duodenum in vivo when compared to the duodenal organoids used to create the chips. Because fluids flowing through the lumen of the Intestine Chip can be collected continuously, sequential analysis of fluid samples can be used to quantify nutrient digestion, mucus secretion and establishment of intestinal barrier function over a period of multiple days in vitro. The Intestine Chip therefore may be useful as a research tool for applications where normal intestinal function is crucial, including studies of metabolism, nutrition, infection, and drug pharmacokinetics, as well as personalized medicine.

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Development of a primary human Small Intestine-on-a-Chip using biopsy-derived organoids

This article argues that because the home is so familiar, it is necessary to make it strange, or defamiliarize it, in order to open its design space. Critical approaches to technology design are of both practical and social importance in the home. Home appliances are loaded with cultural associations such as the gendered division of domestic labor that are easy to overlook. Further, homes are not the same everywhere---even within a country. Peoples' aspirations and desires differ greatly across and between cultures. The target of western domestic technology design is often not the user, but the consumer. Web refrigerators that create shopping lists, garbage cans that let advertisers know what is thrown away, cabinets that monitor their contents and order more when supplies are low are central to current images of the wireless, digital home of the future. Drawing from our research in the United States, the United Kingdom, and Asia, we provide three different narratives of defamiliarization. A historical reading of American kitchens provides a lens with which to scrutinize new technologies of domesticity, an ethnographic account of an extended social unit in England problematizes taken-for-granted domestic technologies, and a comparative ethnography of the role of information and communication technologies in the daily lives of urban Asia's middle classes reveals the ways in which new technologies can be captured and domesticated in unexpected ways. In the final section of the article, we build on these moments of defamiliarization to suggest a broad set of challenges and strategies for design in the home.

/pdf/making-by-making-strange-defamiliarization-and-the-design-of-4ve4e886iu.pdf

Making by making strange: Defamiliarization and the design of domestic technologies

We trace how cultural probes have been adopted and adapted by the HCI community. The flexibility of probes has been central to their uptake, resulting in a proliferation of divergent uses and derivatives. The varying patterns of adaptation of the probes reveal important underlying issues in HCI, suggesting underacknowledged disagreements about valid interpretation and the relationship between methods and their underlying methodology. With this analysis, we aim to clarify discussions around probes, and, more importantly, around how we define and evaluate methods in HCI, especially those grounded in unfamiliar conceptions of how research should be done.

/pdf/how-hci-interprets-the-probes-3pmixiabyn.pdf

How HCI interprets the probes

There has been a growing need for in vitro models of neurodegenerative diseases such as Alzheimer's disease that would enable a better understanding of etiology and faster development of treatment strategies. However, meeting this demand has been held back by the limited ability to mimic the in vivo microenvironment in an in vitro system. Here, we developed a microfluidic chip based on three-dimensional (3D) neurospheroids that more closely mimics the in vivo brain microenvironment by providing a constant flow of fluid that is readily observed in the interstitial space of the brain. Uniform neurospheroids, with cell–cell interactions and contacts in all directions, were formed in concave microwell arrays, and a slow interstitial level of flow was maintained using an osmotic micropump system. Using this platform, we investigated the effect of flow on neurospheroid size, neural network, and neural differentiation. Neurospheroids cultured with flow were larger and formed more robust and complex neural networks than those cultured under static conditions, suggesting an effect of the interstitial level of slow and diffusion-dominant flow on continuous nutrient, oxygen, and cytokine transport and removal of metabolic wastes. We also tested the toxic effects of amyloid-β, which is generally considered to be the major contributor in Alzheimer's disease. Amyloid-β treatment via an osmotic micropump significantly reduced the viability of neurospheroids and caused a significantly more destruction of neural networks, compared to the amyloid-β treatment under static conditions. By adding in vivo-like microenvironments, we propose this 3D culture-based microfluidic chip as an in vitro brain model for neurodegenerative disease and high-throughput drug screening.

Three-dimensional brain-on-a-chip with an interstitial level of flow and its application as an in vitro model of Alzheimer's disease

Cell encapsulation via microtechnologies

The technology biography combines and adapts a number of qualitative data collection techniques to focus on past, present and possible future domestic technologies. Processes, concerns and problems of domestic life are identified in order to develop illustrative product suggestions to inspire or provoke designers.

Technology biographies: field study techinques for home use product development

Size-controllable networked neurospheres as a 3D neuronal tissue model for Alzheimer's disease studies.

https://www.cell.com/trends/biotechnology/pdf/S0167-7799(15)00193-6.pdf

Jisoo Park

Papers

Three-dimensional brain-on-a-chip with an interstitial level of flow and its application as an in vitro model of Alzheimer's disease

Cell encapsulation via microtechnologies

Technology biographies: field study techinques for home use product development

Size-controllable networked neurospheres as a 3D neuronal tissue model for Alzheimer's disease studies.

Central Nervous System and its Disease Models on a Chip