J
Joachim Wegener
Researcher at University of Regensburg
Publications - 126
Citations - 6232
Joachim Wegener is an academic researcher from University of Regensburg. The author has contributed to research in topics: Quartz crystal microbalance & Electric cell-substrate impedance sensing. The author has an hindex of 41, co-authored 121 publications receiving 5812 citations. Previous affiliations of Joachim Wegener include Rensselaer Polytechnic Institute & University of Münster.
Papers
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Electric cell-substrate impedance sensing (ECIS) as a noninvasive means to monitor the kinetics of cell spreading to artificial surfaces.
TL;DR: It is demonstrated that high-frequency capacitance measurements are most suited to follow the increasing surface coverage of the electrode due to cell spreading, and the excellent time resolution of the ECIS technique allowed an in-depth analysis of cell spreading kinetics under various experimental conditions.
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Electrical wound-healing assay for cells in vitro
TL;DR: In this approach, cells growing on small electrodes and monitored by using electric cell-substrate impedance sensing are subjected to currents, resulting in severe electroporation and subsequent cell death.
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Bioelectrical impedance assay to monitor changes in cell shape during apoptosis.
TL;DR: It is observed that disassembly of barrier-forming tight junctions precedes changes in cell-substrate contacts and correlates strongly with the time course of protease activation.
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Hydrocortisone reinforces the blood-brain barrier properties in a serum free cell culture system.
Dirk Hoheisel,Thorsten Nitz,Helmut Franke,Joachim Wegener,Ansgar Hakvoort,Thomas Tilling,Hans-Joachim Galla +6 more
TL;DR: A serum-free cell culture model is established and it is found that hydrocortisone is a potent stimulator for the formation of barrier properties and decreased cell monolayer permeability for sucrose down to 5 x 10(-7) cm/s, which is close to the in vivo value.
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A nanogel for ratiometric fluorescent sensing of intracellular pH values.
Hongshang Peng,Hongshang Peng,Judith A. Stolwijk,Lining Sun,Joachim Wegener,Otto S. Wolfbeis +5 more
TL;DR: A ratiometric fluorescent nanogel can sense pH over a range that is applicable to physiological studies and can be easily prepared and made pH-responsive by addition of a pH probe and a FRET system that utilizes the gel to hold dyes in close proximity.