J
Joanna Buchthal
Researcher at Massachusetts Institute of Technology
Publications - 11
Citations - 1144
Joanna Buchthal is an academic researcher from Massachusetts Institute of Technology. The author has contributed to research in topics: Gene drive & Population. The author has an hindex of 7, co-authored 11 publications receiving 909 citations. Previous affiliations of Joanna Buchthal include Wyss Institute for Biologically Inspired Engineering.
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Journal ArticleDOI
Comparison of Cas9 activators in multiple species
Alejandro Chavez,Alejandro Chavez,Marcelle Tuttle,Benjamin W. Pruitt,Ben Ewen-Campen,Raj Chari,Dmitry Ter-Ovanesyan,Dmitry Ter-Ovanesyan,Sabina J Haque,Ryan J Cecchi,Emma J. K. Kowal,Joanna Buchthal,Benjamin E. Housden,Norbert Perrimon,Norbert Perrimon,James J. Collins,George M. Church,George M. Church +17 more
TL;DR: This work compares Cas9 activator systems and examines their ability to induce robust gene expression in several human, mouse, and fly cell lines, and assesses the role of cooperativity in maximizing gene expression.
Journal ArticleDOI
Cas9 gRNA engineering for genome editing, activation and repression
Samira Kiani,Alejandro Chavez,Alejandro Chavez,Marcelle Tuttle,Richard N. Hall,Raj Chari,Dmitry Ter-Ovanesyan,Dmitry Ter-Ovanesyan,Jason Qian,Jason Qian,Benjamin W. Pruitt,Jacob Beal,Suhani Vora,Suhani Vora,Suhani Vora,Joanna Buchthal,Emma J. K. Kowal,Mohammad R. Ebrahimkhani,James J. Collins,Ron Weiss,George M. Church,George M. Church +21 more
TL;DR: In this paper, the length of Cas9-associated guide RNA (gRNA) was altered to control Cas9 nuclease activity and simultaneously perform genome editing and transcriptional regulation with a single Cas9 protein.
Journal ArticleDOI
Daisy-chain gene drives for the alteration of local populations.
Charleston Noble,John Min,Jason Olejarz,Joanna Buchthal,Alejandro Chavez,Andrea L. Smidler,Erika A. DeBenedictis,George M. Church,Martin A. Nowak,Kevin M. Esvelt +9 more
TL;DR: A self-exhausting form of CRISPR-based drive system comprising genetic elements arranged in a daisy chain such that each drives the next, called a “daisy-chain drive.”
Comparison of Cas9 activators in multiple species
Alejandro Chavez,Marcelle Tuttle,Benjamin W. Pruitt,Ben Ewen-Campen,Raj Chari,Dmitry Ter-Ovanesyan,Sabina J Haque,Ryan J Cecchi,Emma J. K. Kowal,Joanna Buchthal,Benjamin E. Housden,Norbert Perrimon,George M. Church,James J. Collins +13 more
TL;DR: Several groups have generated programmable transcription factors based on the versatile Cas9 protein, yet their relative potency and effectiveness across various cell types and species remain
Cas9 gRNA engineering for genome editing, activation and repression
Alejandro Chavez,Marcelle Tuttle,Raj Chari,Dmitry Ter-Ovanesyan,Jason Qian,Benjamin W. Pruitt,Joanna Buchthal,George M. Church,Samira Kiani,Richard N. Hall,Jacob Beal,Suhani Vora,Emma J. K. Kowal,Mohammad R. Ebrahimkhani,James J. Collins,Ron Weiss +15 more
TL;DR: It is demonstrated that by altering the length of Cas9-associated guide RNA (gRNA) it is able to control Cas9 nuclease activity and simultaneously perform genome editing and transcriptional regulation with a single Cas9 protein.