R
Raj Chari
Researcher at Harvard University
Publications - 89
Citations - 5049
Raj Chari is an academic researcher from Harvard University. The author has contributed to research in topics: Biology & Gene. The author has an hindex of 36, co-authored 68 publications receiving 4347 citations. Previous affiliations of Raj Chari include Massachusetts Institute of Technology & BC Cancer Research Centre.
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Journal ArticleDOI
Comparison of Cas9 activators in multiple species
Alejandro Chavez,Alejandro Chavez,Marcelle Tuttle,Benjamin W. Pruitt,Ben Ewen-Campen,Raj Chari,Dmitry Ter-Ovanesyan,Dmitry Ter-Ovanesyan,Sabina J Haque,Ryan J Cecchi,Emma J. K. Kowal,Joanna Buchthal,Benjamin E. Housden,Norbert Perrimon,Norbert Perrimon,James J. Collins,George M. Church,George M. Church +17 more
TL;DR: This work compares Cas9 activator systems and examines their ability to induce robust gene expression in several human, mouse, and fly cell lines, and assesses the role of cooperativity in maximizing gene expression.
Journal ArticleDOI
Unraveling CRISPR-Cas9 genome engineering parameters via a library-on-library approach
TL;DR: An in vivo library-on-library methodology to simultaneously assess single guide RNA (sgRNA) activity across ∼1,400 genomic loci is developed and provides a generalizable framework to study nucleic acid–nucleic acid interactions and biochemistry in high throughput.
Journal ArticleDOI
Cas9 gRNA engineering for genome editing, activation and repression
Samira Kiani,Alejandro Chavez,Alejandro Chavez,Marcelle Tuttle,Richard N. Hall,Raj Chari,Dmitry Ter-Ovanesyan,Dmitry Ter-Ovanesyan,Jason Qian,Jason Qian,Benjamin W. Pruitt,Jacob Beal,Suhani Vora,Suhani Vora,Suhani Vora,Joanna Buchthal,Emma J. K. Kowal,Mohammad R. Ebrahimkhani,James J. Collins,Ron Weiss,George M. Church,George M. Church +21 more
TL;DR: In this paper, the length of Cas9-associated guide RNA (gRNA) was altered to control Cas9 nuclease activity and simultaneously perform genome editing and transcriptional regulation with a single Cas9 protein.
Journal ArticleDOI
Oncogene mutations, copy number gains and mutant allele specific imbalance (MASI) frequently occur together in tumor cells.
Junichi Soh,Junichi Soh,Naoki Okumura,William W. Lockwood,Hiromasa Yamamoto,Hisayuki Shigematsu,Wei Zhang,Raj Chari,David S. Shames,David S. Shames,Ximing Tang,Calum MacAulay,Marileila Varella-Garcia,Tõnu Vooder,Ignacio I. Wistuba,Stephen Lam,Rolf A. Brekken,Shinichi Toyooka,John D. Minna,Wan L. Lam,Adi F. Gazdar +20 more
TL;DR: The frequent finding of mutations, CNGs and MASI occurring together in tumor cells indicates that these three genetic alterations, acting together, may have a greater role in the development or maintenance of the malignant phenotype than any individual alteration.
Journal ArticleDOI
Whole-genome analysis and HLA genotyping of enteropathy-type T-cell lymphoma reveals 2 distinct lymphoma subtypes.
Ronald J. deLeeuw,Andreas Zettl,Erdwine Klinker,Eugenia Haralambieva,Magan Trottier,Raj Chari,Yong Ge,Randy D. Gascoyne,Andreas Chott,Hans–Konrad Müller–Hermelink,Wan L. Lam +10 more
TL;DR: Contrary to current clinical classification, ETL comprises 2 morphologically, clinically, and genetically distinct lymphoma entities; type 2 ETL may not be associated with celiac disease.