Johanna Geuder

TL;DR: This work systematically evaluates experimental conditions of this protocol and finds that adding polyethylene glycol considerably increases sensitivity by enhancing cDNA synthesis and using Terra polymerase increases efficiency due to a more even cDNA amplification that requires less sequencing of libraries.

TL;DR: In this article, the authors reported the generation of primate induced pluripotent stem cells (iPSCs) from urine samples and showed that suspension-sendai virus transduction of reprogramming factors into urinary cells efficiently generates integration-free iPSCs which maintain their pluripotency under feeder-free culture conditions.

TL;DR: In this paper , the authors optimize and validate prime-seq, an early barcoding bulk RNA-seq method, and show that it performs equivalently to TruSeq, but is four times more cost-efficient due to almost 50 times cheaper library costs.

TL;DR: This study introduces a novel and efficient approach to generate iPSCs non-invasively from primate urine, which will allow to extend the zoo of species available for a comparative approach to molecular and cellular phenotypes.

TL;DR: Adding polyethylene glycol considerably increases sensitivity and using Terra polymerase increases efficiency due to a more even cDNA amplification that requires less sequencing of libraries, combined with other improvements to a new scRNA-seq library protocol, which is shown to be the most sensitive and one of the most efficient and flexible sc RNA-seq methods to date.