A new method is presented for quantifying proteomic and metabolomic profile data by liquid chromatography−mass spectrometry (LC−MS) with electrospray ionization. This biotechnology provides differential expression measurements and enables the discovery of biological markers (biomarkers). Work presented here uses human serum but is applicable to any fluid or tissue. The approach relies on linearity of signal versus molecular concentration and reproducibility of sample processing. There is no use of isotopic labeling or chemically similar standard materials. Linear standard curves are reported for a variety of compounds introduced into human serum. As a measure of analytical reproducibility for proteome and metabolome sampling, median coefficients of variation of 25.7 and 23.8%, respectively, were determined for ∼3400 molecular ions (not counting their numerous isotopes) from 25 independently processed human serum samples, corresponding to a total of 85 000 individual molecular ion measurements.

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Quantification of proteins and metabolites by mass spectrometry without isotopic labeling or spiked standards

An improved procedure for the methylation analysis of oligosaccharides and polysaccharides

Using Cellosolve as a solubilizing additive, up to 5.84 ml of aqueous bicarbonate solutions and essentially equivalent amounts of urine and plasma containing carbon-14- sodium carbonate can be assayed by liquid scintillation counting techniques. Of particular significance is the realization of determining carbon-14 activity with relatively high efficiency in the important biological fluids. (auth)

Determination of carbon-14 in aqueous bicarbonate solutions by liquid scintillation counting techniques. application to biological fluids

The suitability of urethane anesthesia for physiopharmacological investigations is reviewed. Total dose administered and route of administration are recognized as factors having a great influence on both resting parameters and biological responses to drugs. A peculiar characteristic of urethane is represented by its ability to induce a surgical plane of anesthesia without affecting neurotransmission in various subcortical areas and the peripheral nervous system. This makes urethane a suitable general anesthetic for studying neural function in both central and peripheral nervous systems and accounts for the preservation of a number of reflex responses in urethane-anesthetized animals.

Suitability of urethane anesthesia for physiopharmacological investigations in various systems. Part 1: General considerations.

(1980). Chlorinated Phenols: Occurrence, Toxicity, Metabolism, And Environmental Impact. CRC Critical Reviews in Toxicology: Vol. 7, No. 1, pp. 1-35.

Chlorinated Phenols: Occurrence, Toxicity, Metabolism, And Environmental Impact

In vivo determinations of lean body mass from K40 measurements and the relationship of these values to motor aptitude in preadolescent boys

In vivo method for the simultaneous determination of potassium and sodium depletion

The synthesis of a new astringent, aluminum methionate, is reported. Extensive comparative protein precipitation tests for astringency indicate that this salt is more effective as an astringent than other common astringent agents tested, and thus may be applied to improve perspiration retarding or inhibiting preparations. Cloth tests indicate that aluminum methionate is harmless to fabric.

A comparison of a new astringent agent with such agents now commonly used.

Radioactive tracer techniques and statistical methods have been applied to study the uniformity of distribution of several additive phosphorus-32 labeled compounds in a lactose excipient in tablet matrices. Certain physical properties of the additive and excipient powders, the duration of mixing, methods of granulation and drying, solubility of the additive in the granulating agent, additive migration, and other factors, were studied for their effect on the uniformity of additive distribution.

The Uniformity of Distribution of Phosphorus Compounds in Tablet Matrices Using Radioactive Tracer Techniques

A preliminary in vitro study to determine the effect of alcohol vapor on the surface tension of water was carried out. A similar determination of the effect of alcohol breath was tried with positive results, which suggested a method for determining the effect of deodorants on the elimination of alcohol breath.

John E. Christian

Papers

In vivo determinations of lean body mass from K40 measurements and the relationship of these values to motor aptitude in preadolescent boys

In vivo method for the simultaneous determination of potassium and sodium depletion

A comparison of a new astringent agent with such agents now commonly used.

The Uniformity of Distribution of Phosphorus Compounds in Tablet Matrices Using Radioactive Tracer Techniques

A Proposed Method for Determining the Effect of Deodorants on the Elimination of Alcohol Breath