A new method is presented for quantifying proteomic and metabolomic profile data by liquid chromatography−mass spectrometry (LC−MS) with electrospray ionization. This biotechnology provides differential expression measurements and enables the discovery of biological markers (biomarkers). Work presented here uses human serum but is applicable to any fluid or tissue. The approach relies on linearity of signal versus molecular concentration and reproducibility of sample processing. There is no use of isotopic labeling or chemically similar standard materials. Linear standard curves are reported for a variety of compounds introduced into human serum. As a measure of analytical reproducibility for proteome and metabolome sampling, median coefficients of variation of 25.7 and 23.8%, respectively, were determined for ∼3400 molecular ions (not counting their numerous isotopes) from 25 independently processed human serum samples, corresponding to a total of 85 000 individual molecular ion measurements.

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Quantification of proteins and metabolites by mass spectrometry without isotopic labeling or spiked standards

An improved procedure for the methylation analysis of oligosaccharides and polysaccharides

Using Cellosolve as a solubilizing additive, up to 5.84 ml of aqueous bicarbonate solutions and essentially equivalent amounts of urine and plasma containing carbon-14- sodium carbonate can be assayed by liquid scintillation counting techniques. Of particular significance is the realization of determining carbon-14 activity with relatively high efficiency in the important biological fluids. (auth)

Determination of carbon-14 in aqueous bicarbonate solutions by liquid scintillation counting techniques. application to biological fluids

The suitability of urethane anesthesia for physiopharmacological investigations is reviewed. Total dose administered and route of administration are recognized as factors having a great influence on both resting parameters and biological responses to drugs. A peculiar characteristic of urethane is represented by its ability to induce a surgical plane of anesthesia without affecting neurotransmission in various subcortical areas and the peripheral nervous system. This makes urethane a suitable general anesthetic for studying neural function in both central and peripheral nervous systems and accounts for the preservation of a number of reflex responses in urethane-anesthetized animals.

Suitability of urethane anesthesia for physiopharmacological investigations in various systems. Part 1: General considerations.

(1980). Chlorinated Phenols: Occurrence, Toxicity, Metabolism, And Environmental Impact. CRC Critical Reviews in Toxicology: Vol. 7, No. 1, pp. 1-35.

Chlorinated Phenols: Occurrence, Toxicity, Metabolism, And Environmental Impact

Studies were made to determine if hyaluronidase, when incorporated into ointments, affected the penetration of medication contained in ointments applied to the skin. Four substances were tested: sulfanilamide, sodium sulfanilamide, sodium iodide, and sodium chloride. Three different ointment bases were used: an oleaginous base, an absorption base, and a washable emulsion type base. The ointments were applied to the intact skin of guinea pigs, and a technique using radioactive isotopes is reported for determining the extent of penetration of the labeled medicaments. It was found that the substances studied showed a limited degree of penetration into the skin from the bases used, but except in the case of sodium iodide the penetration was not appreciably affected by hyaluronidase.

The effect of topically applied hyaluronidase on cutaneous permeability to certain substances.

Inhalation of 2,3,5‐triiodobenzoic acid by normal and sulfur dioxide‐exposed rats

Whole Body Measurements of 131I-Tetracycline as an Index of Skeletal Growth

Five new sulfa compounds possessing possible medicinal value have been synthesized and characterized.

John E. Christian

Papers

The effect of topically applied hyaluronidase on cutaneous permeability to certain substances.

Inhalation of 2,3,5‐triiodobenzoic acid by normal and sulfur dioxide‐exposed rats

Whole Body Measurements of 131I-Tetracycline as an Index of Skeletal Growth

The synthesis of chemotherapeutic agents. III.: The synthesis of certain thio compounds

Determination of thickness of walls of hard gelatin capsules by radioisotopic means