A new method is presented for quantifying proteomic and metabolomic profile data by liquid chromatography−mass spectrometry (LC−MS) with electrospray ionization. This biotechnology provides differential expression measurements and enables the discovery of biological markers (biomarkers). Work presented here uses human serum but is applicable to any fluid or tissue. The approach relies on linearity of signal versus molecular concentration and reproducibility of sample processing. There is no use of isotopic labeling or chemically similar standard materials. Linear standard curves are reported for a variety of compounds introduced into human serum. As a measure of analytical reproducibility for proteome and metabolome sampling, median coefficients of variation of 25.7 and 23.8%, respectively, were determined for ∼3400 molecular ions (not counting their numerous isotopes) from 25 independently processed human serum samples, corresponding to a total of 85 000 individual molecular ion measurements.

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Quantification of proteins and metabolites by mass spectrometry without isotopic labeling or spiked standards

An improved procedure for the methylation analysis of oligosaccharides and polysaccharides

Using Cellosolve as a solubilizing additive, up to 5.84 ml of aqueous bicarbonate solutions and essentially equivalent amounts of urine and plasma containing carbon-14- sodium carbonate can be assayed by liquid scintillation counting techniques. Of particular significance is the realization of determining carbon-14 activity with relatively high efficiency in the important biological fluids. (auth)

Determination of carbon-14 in aqueous bicarbonate solutions by liquid scintillation counting techniques. application to biological fluids

The suitability of urethane anesthesia for physiopharmacological investigations is reviewed. Total dose administered and route of administration are recognized as factors having a great influence on both resting parameters and biological responses to drugs. A peculiar characteristic of urethane is represented by its ability to induce a surgical plane of anesthesia without affecting neurotransmission in various subcortical areas and the peripheral nervous system. This makes urethane a suitable general anesthetic for studying neural function in both central and peripheral nervous systems and accounts for the preservation of a number of reflex responses in urethane-anesthetized animals.

Suitability of urethane anesthesia for physiopharmacological investigations in various systems. Part 1: General considerations.

(1980). Chlorinated Phenols: Occurrence, Toxicity, Metabolism, And Environmental Impact. CRC Critical Reviews in Toxicology: Vol. 7, No. 1, pp. 1-35.

Chlorinated Phenols: Occurrence, Toxicity, Metabolism, And Environmental Impact

A method has been described which determines the rectal absorption rate of a small dose of iodide, using radio-iodine. The radioactive tracer technique made possible a number of determinations for statistical treatment of the data, analyzing the significance of the different amounts absorbed from each base tested.

A study of suppository bases using radio-iodine to compare the rate of absorption from rectal suppositories administered to rats†

Effect of aspirin and ethanol on the gastric mucosa of the rat.

A method for the determination of organically bound iodine by the technique of neutron activation analysis was developed with specific reference to acetrizoic acid. Complete analysis can be carried out in less than 1 hr. The estimated precision would be plus or minus 6.8 and plus or minus 3.97% for 10 and 50% sodium acetrizoate solutions, respectively using the conditions described. (P.C.H.)

John E. Christian

Papers

A study of suppository bases using radio-iodine to compare the rate of absorption from rectal suppositories administered to rats†

Effect of aspirin and ethanol on the gastric mucosa of the rat.

Large Volume Activation Analysis of Organically Bound Iodine Using Isotopic Neutron Sources.

A 4π liquid-scintillation whole-body counter—I. Design, operating characteristics, and calibration☆

In vivo pharmacodynamic evaluation of oral dosage forms by whole body liquid scintillometry.