A new method for determining nucleotide sequences in DNA is described. It is similar to the “plus and minus” method [Sanger, F. & Coulson, A. R. (1975) J. Mol. Biol. 94, 441-448] but makes use of the 2′,3′-dideoxy and arabinonucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase. The technique has been applied to the DNA of bacteriophage ϕX174 and is more rapid and more accurate than either the plus or the minus method.

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DNA sequencing with chain-terminating inhibitors

Serial injection of muramyldipeptides and liposomes enhances the anti-infective activity of muramyldipeptides.

Serial injection of muramyldipeptides and liposomes enhances the anti-infective activity of muramyldipeptides

Therapeutic oligonucleotide analogues which have improved nuclease resistance and improved cellular uptake are provided. Replacement of the normal phosphorodiester inter-sugar linkages found in natural oligomers with four atom linking groups forms unique di- and poly-nucleosides and nucleotides useful in regulating RNA expression and in therapeutics. Methods of synthesis and use are also disclosed.

Backbone modified oligonucleotide analogues

Methods for preparing oligonucleotide analogs which have improved nuclease resistance and improved cellular uptake are provided. In preferred embodiments, the methods involve radical coupling of 3'- and 5'-substituted or 5'- and 3'-subutituted nucleosidic synthons.

Backbone modified oligonucleotide analogs and preparation thereof through radical coupling

A lubricating oil composition formulated with a viscosity index (VI) improver composition including a combination of an ethylene α-olefin copolymer having no greater than 66 mass % of units derived from ethylene, and a linear diblock copolymer including at least one block derived primarily from a vinyl aromatic hydrocarbon monomer, and at least one block derived primarily from diene monomer.

Lubricating oil compositions.

Preparation and Synthetic Utility of Some Organotin Derivatives of nucleosides

C-glycosyl nucleosides. V. Some unexpected observations on the relative stabilities of compounds containing fused five-membered rings with epimerizable substituents.

John G. Moffatt

Papers

Preparation and Synthetic Utility of Some Organotin Derivatives of nucleosides

C-glycosyl nucleosides. V. Some unexpected observations on the relative stabilities of compounds containing fused five-membered rings with epimerizable substituents.

Halo sugar nucleosides. I. Iodination of the primary hydroxyl groups of nucleosides with methyltriphenoxyphosphonium iodide

Enzymatic synthesis of deoxyribonucleic acid. XXXIV. Termination of chain growth by a 2',3'-dideoxyribonucleotide

Synthesis of some pyrimidine 2'-amino-2'-deoxynucleosides.