J
John J. Roberts
Researcher at Institute of Cancer Research
Publications - 42
Citations - 2842
John J. Roberts is an academic researcher from Institute of Cancer Research. The author has contributed to research in topics: DNA & Cisplatin. The author has an hindex of 28, co-authored 42 publications receiving 2806 citations.
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Mechanism of cytotoxicity of anticancer platinum drugs: evidence that cis-diamminedichloroplatinum(II) and cis-diammine-(1,1-cyclobutanedicarboxylato)platinum(II) differ only in the kinetics of their interaction with DNA.
TL;DR: It was concluded that the CBDCA ligand becomes a more labile leaving group once carboplatin has been monoaquated, and both chloro-ligands of cisplatin were shown to leave at similar rates, while certain cell lines were showed to be much more sensitive to DNA bound platinum.
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Interactions between mammalian cell DNA and inorganic platinum compounds. II. Interstrand cross-linking of isolated and cellular DNA by platinum(IV) compounds.
Janet M. Pascoe,John J. Roberts +1 more
TL;DR: It is unlikely that the ability of cis and trans Pt(IV)diammine tetrachloride to cross-link isolated or cellular DNA bears any relationship to their cytotoxic properties, and it is concluded that the differences seen in vitro and in vivo were approximately the same.
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A new cytotoxic, DNA interstrand crosslinking agent, 5-(aziridin-1-yl)-4-hydroxylamino-2-nitrobenzamide, is formed from 5-(aziridin-1-yl)-2,4-dinitrobenzamide (CB 1954) by a nitroreductase enzyme in Walker carcinoma cells.
TL;DR: A new compound can form interstrand crosslinks in the DNA of Chinese hamster V79 cells to which it is also highly toxic, findings indicative of the formation by Walker cells of a diffusible toxic metabolite of CB 1954.
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The nitroreductase enzyme in Walker cells that activates 5-(aziridin-1-yl)-2,4-dinitrobenzamide (CB 1954) to 5-(aziridin-1-yl)-4-hydroxylamino-2-nitrobenzamide is a form of NAD(P)H dehydrogenase (quinone) (EC 1.6.99.2).
TL;DR: The reported antagonistic action of the aminoimidazole carboxamides to the antitumour effects of CB 1954 could explain the reported antagonism of these molecules to the cytotoxic DNA interstrand crosslinking agent effects.
Journal ArticleDOI
Interactions between mammalian cell DNA and inorganic platinum compounds. I. DNA interstrand cross-linking and cytotoxic properties of platinum(II) compounds.
Janet M. Pascoe,John J. Roberts +1 more
TL;DR: In this paper, the effects of cis and trans Pt(II)-diammine dichloride on the colony forming ability of HeLa cells have been measured, showing that more of the trans compound was bound to DNA than cis when cell survival was reduced by the same amount, suggesting there was a real difference in the mechanisms of action of the two isomers.