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John K. G. Kramer

Researcher at Agriculture and Agri-Food Canada

Publications -  135
Citations -  7842

John K. G. Kramer is an academic researcher from Agriculture and Agri-Food Canada. The author has contributed to research in topics: Conjugated linoleic acid & Fatty acid. The author has an hindex of 46, co-authored 135 publications receiving 7413 citations. Previous affiliations of John K. G. Kramer include University of Guelph.

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Evaluating acid and base catalysts in the methylation of milk and rumen fatty acids with special emphasis on conjugated dienes and total trans fatty acids

TL;DR: No single method or combination of methods could adequately prepare FAME from all lipid classes in milk or rumen lipids, and not affect the conjugated dienes.
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Combining results of two GC separations partly achieves determination of all cis and trans 16:1, 18:1, 18:2 and 18:3 except CLA isomers of milk fat as demonstrated using Ag-ion SPE fractionation.

TL;DR: The present method takes advantage of differences in the relative elution times between different types of FAs to determine most of the geometric and positional isomers of 16:1, 18:2 and 18:3 without a prior silver-ion separation in total milk fat.
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The effect of conjugated linoleic acid on fat to lean repartitioning and feed conversion in pigs

TL;DR: The data suggest CLA can be used as a repartioning agent in pigs and that further research to optimize the repartitioning effect is warranted.
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Methods for analysis of conjugated linoleic acids and trans-18:1 isomers in dairy fats by using a combination of gas chromatography, silver-ion thin-layer chromatography/gas chromatography, and silver-ion liquid chromatography.

TL;DR: Improved techniques are further required to evaluate the CLA profile in monogastric animals fed commercial CLA preparations for CLA enrichment of animal products and to help design experimental diets to increase the level of the desired CLA isomers in dairy fats.
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Distributions of conjugated linoleic acid (CLA) isomers in tissue lipid classes of pigs fed a commercial CLA mixture determined by gas chromatography and silver ion-high-performance liquid chromatography.

TL;DR: The preferential accumulation of the 11 cis,13 trans-18∶2 into cardiac lipids, and in particular the major phospholipid in the inner mitochondrial membrane, DPG, in both heart and liver, appears unique and may be of concern.