M
Michael P. Washburn
Researcher at University of Kansas
Publications - 299
Citations - 35409
Michael P. Washburn is an academic researcher from University of Kansas. The author has contributed to research in topics: Chromatin & RNA polymerase II. The author has an hindex of 81, co-authored 296 publications receiving 32468 citations. Previous affiliations of Michael P. Washburn include Stowers Institute for Medical Research & Syngenta.
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Large-scale analysis of the yeast proteome by multidimensional protein identification technology.
TL;DR: MudPIT was applied to the proteome of the Saccharomyces cerevisiae strain BJ5460 grown to mid-log phase and yielded the largest proteome analysis to date, identifying 131 proteins with three or more predicted transmembrane domains which allowed us to map the soluble domains of many of the integral membrane proteins.
Journal ArticleDOI
An Automated Multidimensional Protein Identification Technology for Shotgun Proteomics
TL;DR: An automated method for shotgun proteomics named MudPIT, which combines multidimensional liquid chromatography with electrospray ionization tandem mass spectrometry, improves the overall analysis of proteomes by identifying proteins of all functional and physical classes.
Journal ArticleDOI
The CRAPome: a contaminant repository for affinity purification–mass spectrometry data
Dattatreya Mellacheruvu,Zachary Wright,Amber L. Couzens,Jean-Philippe Lambert,Nicole St-Denis,Tuo Li,Yana Miteva,Simon Hauri,Mihaela E. Sardiu,Teck Yew Low,Vincentius A. Halim,Vincentius A. Halim,Richard D. Bagshaw,Nina C. Hubner,Abdallah Al-Hakim,Annie Bouchard,Denis Faubert,Damian Fermin,Wade H. Dunham,Marilyn Goudreault,Zhen Yuan Lin,Beatriz Gonzalez Badillo,Tony Pawson,Daniel Durocher,Benoit Coulombe,Ruedi Aebersold,Ruedi Aebersold,Giulio Superti-Furga,Jacques Colinge,Albert J. R. Heck,Hyungwon Choi,Matthias Gstaiger,Shabaz Mohammed,Ileana M. Cristea,Keiryn L. Bennett,Michael P. Washburn,Michael P. Washburn,Brian Raught,Rob M. Ewing,Rob M. Ewing,Anne-Claude Gingras,Alexey I. Nesvizhskii +41 more
TL;DR: The contaminant repository for affinity purification (the CRAPome) is presented and its use for scoring protein-protein interactions is described and aggregating negative controls from multiple AP-MS studies can increase coverage and improve the characterization of background associated with a given experimental protocol.
Journal ArticleDOI
A proteomic view of the Plasmodium falciparum life cycle
Laurence Florens,Michael P. Washburn,J. Dale Raine,Robert M. Anthony,Munira Grainger,J. David Haynes,J. David Haynes,J. Kathleen Moch,Nemone Muster,John B. Sacci,John B. Sacci,David L. Tabb,David L. Tabb,Adam A. Witney,Adam A. Witney,Dirk Wolters,Dirk Wolters,Yimin Wu,Malcolm J. Gardner,Anthony A. Holder,Robert E. Sinden,John R. Yates,John R. Yates,Daniel J. Carucci +23 more
TL;DR: A high-throughput proteomics approach was applied to identify new potential drug and vaccine targets and to better understand the biology of this complex protozoan parasite.
Journal ArticleDOI
Histone H3 methylation by Set2 directs deacetylation of coding regions by Rpd3S to suppress spurious intragenic transcription.
Michael J. Carrozza,Bing Li,Laurence Florens,Tamaki Suganuma,Selene K. Swanson,Kenneth K. Lee,Wei-Jong Shia,Scott Anderson,John R. Yates,Michael P. Washburn,Jerry L. Workman +10 more
TL;DR: Data indicate that Pol II-associated Set2 methylates H3 providing a transcriptional memory which signals for deacetylation of ORFs by Rpd3S, which erases transcription elongation-associated acetylation to suppress intragenic transcription initiation.