Showing papers by "Joke A. Bouwstra published in 2000"
TL;DR: A short description of the lipid organisation in normal human stratum corneum will be given, after which the role the main lipid classes play in the stratum Corneum lipid organisation will be described and a new model, the "sandwich model", will be proposed that describe the localisation of the fluid phases in the Stratum cor pneumonia.
Abstract: The main function of the skin is to protect the body against exogenous substances. The skin barrier is located in the outermost layer of the skin, the stratum corneum. This layer consists of keratin enriched cells embedded in lipid lamellae. These lamellae form the main barrier for diffusion of substances through the skin. In diseased skin the barrier function is often impaired. For a full understanding of the properties of the human skin barrier, insight in the stratum corneum lipid organisation is of great importance. In this paper a short description of the lipid organisation in normal human stratum corneum will be given, after which the role the main lipid classes play in the stratum corneum lipid organisation will be described. In addition the effect of cholesterol sulfate and calcium on the lipid organisation will be discussed. Finally a new model, the "sandwich model", will be proposed that describe the localisation of the fluid phases in the stratum corneum.
191 citations
TL;DR: It is demonstrated that Cer(EOS) and Cer(EOH) are key elements for the molecular organization of the long periodicity lamellar phase in the human stratum corneum.
125 citations
TL;DR: All three skin models provide a promising means for studying the effects of topically applied chemicals, although the observed deviations in tissue homeostasis and barrier properties need to be optimized.
125 citations
TL;DR: Use of a positively charged enhancer may improve Jssof anionic macromolecular penetrants during passive and iontophoretic delivery and can provide mechanistic insight into SC transport pathways.
Abstract: Purpose. To investigate the transdermal delivery of a modelmacromolecule by passive and iontophoretic means following pretreatment withC12-penetration enhancers and to visualise transport across humanstratum corneum (SC) in real time.
32 citations
TL;DR: The increased penetration of Hg into OAz treated skincare samples could not be related to an orthorhombic-hexagonal phase transition, and phase separation of OAz and/or formation of grainboundaries might affect SC permeability, hereby increasing Hgpenetration.
Abstract: Purpose. Investigation of the relationship between changes in humanSC lipid organization induced by N-alkyl-azocycloheptane-2-one andSC permeability to the model compound HgCl2.
19 citations