Jordi Solana

TL;DR: Partition-based graph abstraction (PAGA) provides an interpretable graph-like map of the arising data manifold, based on estimating connectivity of manifold partitions, which preserves the global topology of data, allow analyzing data at different resolutions, and result in much higher computational efficiency of the typical exploratory data analysis workflow.

TL;DR: This work applied single-cell transcriptomics to regenerating planarians and characterized how each cell type in the adult planarian body dynamically responds to regenerative body remodeling at the transcriptomic and cellular levels.

TL;DR: In this article, the authors analyzed the gene expression in freshwater planaria and found conserved expression of epigenetic regulators and demonstrated their requirement for planarian regeneration by knockdown experiments.

TL;DR: The use of Smed-histone-2B RNA interference for genetic ablation of neoblast cells in Schmidtea mediterranea is introduced and data reveal that the regulation of planarian stem cells relies on posttranscriptional regulatory mechanisms and suggest that planarians are an ideal model for this understudied aspect of stem cell biology.

TL;DR: The method is demonstrated by inferring structure-rich cell maps with consistent topology across four hematopoietic datasets, confirm the reconstruction of lineage relations of adult planaria and the zebrafish embryo, benchmark computational performance on a neuronal dataset and detect a biological trajectory in one deep-learning processed image dataset.