Fiona K. Hamey

TL;DR: Partition-based graph abstraction (PAGA) provides an interpretable graph-like map of the arising data manifold, based on estimating connectivity of manifold partitions, which preserves the global topology of data, allow analyzing data at different resolutions, and result in much higher computational efficiency of the typical exploratory data analysis workflow.

TL;DR: Reconstruction of differentiation trajectories reveals dynamic expression changes associated with early lymphoid, erythroid, and granulocyte-macrophage differentiation as well as estimating absolute messenger RNA levels per cell.

TL;DR: Single-cell RNA-sequencing is applied to a population of hematopoietic cells in zebrafish as they undergo thrombocyte lineage commitment, reconstructing their developmental chronology computationally to place each cell along a continuum from stem cell to mature cell, refining the traditional lineage tree.

TL;DR: It is shown that a continuum of hemopoietic progenitor cells from human cord blood execute lymphoid and myeloid differentiation, rather than only uni-lineage progenitors’ being present downstream of stem cells.

TL;DR: In this article, the authors used single-cell RNA sequencing to identify a common basophil/mast cell bone marrow progenitor and characterized its molecular profile at the single cell level.