Joseph Groom

TL;DR: A next-generation sequencing method that rapidly and cost efficiently reveals the genome-wide locations of 4mC for bacterial species with an available assembled reference genome to study the methylation of a member of the hyperthermophilc genus, Caldicellulosiruptor.

TL;DR: A new expression vector was constructed for C. bescii and was able, for the first time, to express significant quantities of full-length protein in vivo in the native host, allowing the study of the mechanism of protein glycosylation in this thermophile.

TL;DR: An inventory of genes potentially encoding Ln3+-dependent enzymes, closely related to the previously characterized XoxF and ExaF/PedH enzymes are compiled, demonstrating a range of catalytic properties and substrate and cofactor specificities.

TL;DR: The TonB-dependent receptor LanA is the first lanthanum receptor identified in a methanotroph and advances the knowledge of microbe-metal interactions in environmental niches that impact atmospheric methane levels, and are thus relevant to climate change.

TL;DR: The use of C. hydrothermalis as a host for homologous and heterologous expression of enzymes important for biomass deconstruction will enable the identification of enzymes that contribute to the special ability of these bacteria to degrade complex lignocellulosic substrates as well as facilitate the construction of strains to improve and extend their substrate utilization capabilities.