J
Juerg Friedrich Tschopp
Researcher at Salk Institute for Biological Studies
Publications - 13
Citations - 1363
Juerg Friedrich Tschopp is an academic researcher from Salk Institute for Biological Studies. The author has contributed to research in topics: Pichia pastoris & Yeast. The author has an hindex of 7, co-authored 13 publications receiving 1343 citations.
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Expression of the lacZ gene from two methanol-regulated promoters in Pichia pastoris
TL;DR: Two DNA fragments containing putative control regions regulating the expression of the alcohol oxidase and dihydroxy-acetone synthase genes from the methylotrophic yeast Pichia pastoris were used in the construction of vectors for theexpression of the Escherichia coli lacZ gene.
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High–Level Expression and Efficient Assembly of Hepatitis B Surface Antigen in the Methylotrophic Yeast, Pichia Pastoris
James M. Cregg,Juerg Friedrich Tschopp,Cathy A. Stillman,Robert Steven Siegel,M. Akong,William Scot Craig,Richard Gordon Buckholz,Knut Madden,P. A. Kellaris,Geneva Ruth Davis,B. L. Smiley,J. Cruze,R. Torregrossa,G. Velic ecedil,lebi,Gregory Patrick Thill +15 more
TL;DR: The methylotrophic industrial yeast, Pi–chia pastoris, has been developed as a host system for the large–scale production of heterologous proteins, and the synthesis of hepatitis B surface antigen (HBsAg) is described.
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Size distribution and general structural features of N-linked oligosaccharides from the methylotrophic yeast, Pichia pastoris.
TL;DR: The secreted glycoproteins of Pichia pastoris contain more than 35% of their N‐linked oligosaccharides as structures smaller than Man14GlcNAc2, and this finding increases the utility of P. pastoris as a host for the production of heterologous gly coproteins.
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High-Level Secretion of Glycosylated Invertase in the Methylotrophic Yeast, Pichia Pastoris
TL;DR: SUC2 gene expression under the control of the alcohol oxidase (AOX1) promoter resulted in efficient secretion of biologically active invertase into the periplasmic space and growth medium of P. pastoris, giving the methylotrophic yeast a Suc+ phenotype.