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James M. Cregg

Researcher at Keck Graduate Institute of Applied Life Sciences

Publications -  108
Citations -  20715

James M. Cregg is an academic researcher from Keck Graduate Institute of Applied Life Sciences. The author has contributed to research in topics: Pichia pastoris & Peroxisome. The author has an hindex of 54, co-authored 108 publications receiving 19232 citations. Previous affiliations of James M. Cregg include Salk Institute for Biological Studies & Henkel.

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Guidelines for the use and interpretation of assays for monitoring autophagy

Daniel J. Klionsky, +1287 more
- 01 Apr 2012 - 
TL;DR: These guidelines are presented for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes.
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Guidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotes

Daniel J. Klionsky, +235 more
- 16 Feb 2008 - 
TL;DR: A set of guidelines for the selection and interpretation of the methods that can be used by investigators who are attempting to examine macroautophagy and related processes, as well as by reviewers who need to provide realistic and reasonable critiques of papers that investigate these processes are presented.
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Heterologous protein expression in the methylotrophic yeast Pichia pastoris

TL;DR: This paper reviews the P. pastoris expression system: how it was developed, how it works, and what proteins have been produced and describes new promoters and auxotrophic marker/host strain combinations which extend the usefulness of the system.
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Recent advances in the expression of foreign genes in Pichia pastoris

TL;DR: The Pichia pastoris heterologous gene expression system has been utilized to produce attractive levels of a variety of intracellular and extracellular proteins of interest and improvements in understanding and application have improved its utility even further.