Jun Hyoung Lee

TL;DR: Novel ATFs capable of reprogramming innate gene expression circuits in Escherichia coli induced various phenotypic changes in E. coli and selected for industrially important traits, such as resistance to heat shock, osmotic pressure and cold shock, indicating that novel ATFs are powerful tools for the phenotyping engineering of microorganisms and can facilitate microbial functional genomic studies.

TL;DR: A genome-engineering tool for determining essential genes and minimizing bacterial genomes is developed and two large pools of independent transposon mutants in Escherichia coli are made using modified Tn5 transposons with two different selection markers.

TL;DR: This work sequenced and assembled the ginseng transcriptome de novo and characterized two UDP-glycosyltransferases (PgUGTs), indicating that these two UGTs are key enzymes for the synthesis of ginsenosides and provide a method for producing specific ginsene biosynthesis through yeast fermentation.

TL;DR: This result demonstrates that the elimination of genes unnecessary for cell growth can increase the productivity of an industrial strain, most likely by reducing the metabolic burden and improving the metabolic efficiency of cells.

TL;DR: Adaptive laboratory evolution is deployed to re-optimise growth performance and show transcriptome and translatome-wide remodeling of the organism that orchestrate metabolism and growth.