J
Jun Li
Researcher at University of Alberta
Publications - 12
Citations - 255
Jun Li is an academic researcher from University of Alberta. The author has contributed to research in topics: Electrospray ionization & Membrane protein. The author has an hindex of 7, co-authored 10 publications receiving 190 citations.
Papers
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Journal ArticleDOI
The Peptidisc, a simple method for stabilizing membrane proteins in detergent-free solution.
Michael Carlson,John W. Young,Zhiyu Zhao,Lucien Fabre,Daniel Jun,Daniel Jun,Jianing Li,Jun Li,Harveer Singh Dhupar,Irvin Wason,Allan Mills,J. Thomas Beatty,John S. Klassen,Isabelle Rouiller,Franck Duong +14 more
TL;DR: The peptidisc method is rapid and cost-effective, and it may emerge as a universal tool for high-throughput stabilization of membrane proteins to advance modern biological studies.
Journal ArticleDOI
Protein-glycolipid interactions studied in vitro using ESI-MS and nanodiscs: insights into the mechanisms and energetics of binding.
Ling Han Han,Elena N. Kitova,Jun Li,Sanaz Nikjah,Hong Lin,Benjamin Pluvinage,Alisdair B. Boraston,John S. Klassen +7 more
TL;DR: The results of the ESI-MS measurements reveal that proteins bind reversibly to ND-bound GLs and that proteins possessing multiple ligand binding sites are able to interact with GLs originating from different NDs.
Journal ArticleDOI
Picodiscs for Facile Protein-Glycolipid Interaction Analysis
Aneika C. Leney,Reza Rezaei Darestani,Jun Li,Sanaz Nikjah,Elena N. Kitova,Chunxia Zou,Christopher W. Cairo,Zi Jian Xiong,Gilbert G. Privé,Gilbert G. Privé,John S. Klassen +10 more
TL;DR: A powerful new assay is described that combines electrospray ionization mass spectrometry (ESI-MS) and picodiscs, which are composed of human sphingolipid activator protein saposin A and a small number of phospholipids, to display glycolipids in a lipid environment for protein-glycolipid interaction studies in aqueous solution.
Journal ArticleDOI
Screening Glycolipids Against Proteins in Vitro Using Picodiscs and Catch-and-Release Electrospray Ionization-Mass Spectrometry
Jun Li,Xuxin Fan,Elena N. Kitova,Chunxia Zou,Christopher W. Cairo,Luiz Eugenio,Luiz Eugenio,Kenneth K.-S. Ng,Kenneth K.-S. Ng,Zi Jian Xiong,Gilbert G. Privé,Gilbert G. Privé,John S. Klassen +12 more
TL;DR: A comparison of the present results with data obtained with the CaR-ESI-MS assay implemented using nanodiscs (NDs) revealed that the PDs exhibited similar or superior performance to NDs for protein-GL binding measurements.
Peer ReviewDOI
Author response: The Peptidisc, a simple method for stabilizing membrane proteins in detergent-free solution
Michael Carlson,John W. Young,Zhiyu Zhao,Lucien Fabre,Daniel Jun,Daniel Jun,Jianing Li,Jun Li,Harveer Singh Dhupar,Irvin Wason,Allan Mills,J. Thomas Beatty,John S. Klassen,Isabelle Rouiller,Franck Duong +14 more