Z
Zhiyu Zhao
Researcher at University of British Columbia
Publications - 5
Citations - 183
Zhiyu Zhao is an academic researcher from University of British Columbia. The author has contributed to research in topics: Membrane protein & Interactome. The author has an hindex of 2, co-authored 5 publications receiving 110 citations.
Papers
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Journal ArticleDOI
The Peptidisc, a simple method for stabilizing membrane proteins in detergent-free solution.
Michael Carlson,John W. Young,Zhiyu Zhao,Lucien Fabre,Daniel Jun,Daniel Jun,Jianing Li,Jun Li,Harveer Singh Dhupar,Irvin Wason,Allan Mills,J. Thomas Beatty,John S. Klassen,Isabelle Rouiller,Franck Duong +14 more
TL;DR: The peptidisc method is rapid and cost-effective, and it may emerge as a universal tool for high-throughput stabilization of membrane proteins to advance modern biological studies.
Journal ArticleDOI
Profiling the Escherichia coli membrane protein interactome captured in Peptidisc libraries
Michael Carlson,R. Greg Stacey,John W. Young,Irvinder Singh Wason,Zhiyu Zhao,David G Rattray,Nichollas E. Scott,Craig H. Kerr,Mohan Babu,Leonard J. Foster,Franck Duong Van Hoa +10 more
TL;DR: The peptidisc workflow applied to the proteomic field is emerging as a promising novel approach to characterize membrane protein interactions under native expression conditions and without genetic manipulation.
Peer ReviewDOI
Author response: The Peptidisc, a simple method for stabilizing membrane proteins in detergent-free solution
Michael Carlson,John W. Young,Zhiyu Zhao,Lucien Fabre,Daniel Jun,Daniel Jun,Jianing Li,Jun Li,Harveer Singh Dhupar,Irvin Wason,Allan Mills,J. Thomas Beatty,John S. Klassen,Isabelle Rouiller,Franck Duong +14 more
Journal ArticleDOI
His-Tagged Peptidiscs Enable Affinity Purification of the Membrane Proteome for Downstream Mass Spectrometry Analysis.
John W. Young,Irvinder Singh Wason,Zhiyu Zhao,David G Rattray,Leonard J. Foster,Franck Duong Van Hoa +5 more
TL;DR: A His-tagged version of the peptidisc peptide scaffold is employed to enrich the reconstituted membrane proteome by affinity chromatography, which reduces the sample complexity by depleting ribosomal and soluble proteins that often cosediment with cellular membranes.
Peer ReviewDOI
Author response: Profiling the Escherichia coli membrane protein interactome captured in Peptidisc libraries
Michael Carlson,R. Greg Stacey,John W. Young,Irvinder Singh Wason,Zhiyu Zhao,David G Rattray,Nichollas E. Scott,Craig H. Kerr,Mohan Babu,Leonard J. Foster,Franck Duong Van Hoa +10 more