Jung-Eun Kim

TL;DR: Delivery of purified recombinant Cas9 protein and guide RNA into cultured human cells including hard-to-transfect fibroblasts and pluripotent stem cells is delivered and RGEN ribonucleoproteins (RNPs) induce site-specific mutations at frequencies of up to 79%, while reducing off- target mutations associated with plasmid transfection at off-target sites.

TL;DR: Transfected preassembled complexes of purified Cas9 protein and guide RNA into plant protoplasts of Arabidopsis thaliana, tobacco, lettuce and rice and achieved targeted mutagenesis in regenerated plants at frequencies of up to 46%.

TL;DR: It is found that Cpf1 could tolerate single or double mismatches in the 3′ Pam-distal region, but not in the 5′ PAM-proximal region, and off-target effects were completely abrogated by using preassembled, recombinant CpF1 ribonucleoproteins.

TL;DR: A systematic analysis of 17 phenotypes of the mutants in 657 Fusarium graminearum genes encoding putative transcription factors resulted in a database of over 11,000 phenotypes (phenome), providing comprehensive insights into how this cereal pathogen of global significance regulates traits important for growth, development, stress response, pathogenesis, and toxin production.

TL;DR: The results demonstrate that dividing tissue-targeted mutagenesis using RGEN provides an efficient heritable genome engineering method in A. thaliana.